RE: CAM 5.2 from B.Dickinson VS other companie's equivalents
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From: | Alex Brown <AlexB@nayrshire.scot.nhs.uk> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Carole from Quebec,
We used to use B.D.'s CAM 5.2 years ago , but in recent years
have been using an antibody from Novocastra (available from Vector Labs
). It is apparently specific for Moll's Catalogue nos. 8 and 18 . The
clone is 5D3. On human tissue we find the results comparable (as far as
I can tell ) to CAM 5.2 On the 'fact sheet' there are a couple of
references to papers, one of which uses both CAM 5.2 and NCL-5D3. If
this is of any interest I could fax you the data sheet. Just let me
know.
Have a good weekend,
Alex. Brown
Crosshouse Hospital
Kilmarnock, Scotland.
----------
From: Jean-Hugues Deschesnes
To: HistoNet@Pathology.swmed.edu
Subject: CAM 5.2 from B.Dickinson VS other companie's equivalents
Date: 07 January 1999 16:34
Did anybody try to compare the CAM 5.2 from B.D. with another equivalent
product; by
this I intend the same cytok#233#ratines,ie 8 and 18.(on the
specification
sheet,the mol#233#cular weight is respectively 39 and 43 KD).Were the
results as good,as sp#233#cific as the CAM.
We want to have a concentrate because using a prediluted antibody is
very expensive with an automated procedure.Thank you! Carole from
Qu#233#bec.
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