Hi Donna.
We tried to get sc5535 to work on complete and disassociated mouse 
intestinal muscle layer, mouse frozen  gut ( unfixed and fixed-fixed) and on 
pwax sections of same...all negative.
Only one we got to work on each, although it was best on pwax sections, was 
Dako's anti c-kit  A4502 , 1/3-500 dilution factor.
Try it on your frozens: also try pwax sections.
good luck.
Carl 


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