> Without seeing the brown pigment it is hard to say what it is. But, if 
> you are dealing with sections of mouse liver, my first guess would be 
> that the brown material is bile pigment. You are correct in assuming 
> that the blue material is the product formed by the reaction of ferric 
> iron with Perls' reagents.

You say that you see a big difference between treated and untreated 
animals. What sort of treatment have they had?  Let us have some more 
information, and maybe we can help some more.

Paul
Kamloops, BC
Canada

*********************************************************************

>  
>  I did Perls' stain on mouse liver. I undersstand that the bright blue is the target I should focus on. But I also see a lot dark brown stain on them which I don't know what it is. I am eager to know because I do see big difference between treated and untreated animals. I checked my text book and other resouces but could not find the answer. I hope the histonetters here can kindly help me with this.
>   
>  Thanks!
>   
>  Wen
>
>		
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