I am looking for a specific stain for hemoglobin in formalin-fixed,
paraffin-embedded canine tissue. I could either use IHC or a special
stain. Any ideas?

I found a reference for a Puchtler-Rosenthal-Sweat Method for hemoglobin
but it says that it only works in tissue fixed in Zenker's.

Just wondering if anyone has any experience in this before we try it.

Thanks,

Julie

Julie Randolph-Habecker, Ph.D.
Staff Scientist - Manager
Experimental Histopathology Shared Resource
Fred Hutchinson Cancer Research Center
1100 Fairview Ave, N. M5-A803
Seattle WA 98109-1024
206-667-6119
jhabecke@fhcrc.org

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