[Histonet] Need some heat!
It's the Thermo Shandon Para Trimmer. We have two. Ow they get hot!
:)
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Friday, January 20, 2006 11:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 26, Issue 23
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Today's Topics:
1. RE: Cat Hematoxylin (Hofecker, Jennifer L)
2. Need Some Heat! (Mike Cupello)
3. marker of endothelial differentiation in vitro (Mark Adam Tarango)
4. please remove me, thanks! (mason dean)
5. Re: acetylcholinesterase staining (John Kiernan)
6. Re: Counter stain (John Kiernan)
7. Re: Invertebrates in Sediment (John Kiernan)
8. Re: air drying-white sections?? (John Kiernan)
9. Re: Need Some Heat! (gillian.2.brown@gsk.com)
10. job classification (Linda Blazek)
11. Lot to lot antibody validation (Connolly, Brett M)
12. slide storage feedback (Orr, Rebecca)
13. Re: Lot to lot antibody validation (Amy Porter)
14. Cat Hematoxylin (Orr, Rebecca)
15. RE: Need Some Heat! (Rey, Jean-Philippe)
16. RE: Need Some Heat! (Ford Royer)
17. Re: IHC slides and sections (Gayle Callis)
18. Re: Cat Hematoxylin (Gayle Callis)
19. Re: Need Some Heat! (Gayle Callis)
20. Re: Need Some Heat! (Robyn Vazquez)
21. RE: Need Some Heat! (Bartlett, Jeanine)
22. Re: Z fix (Ada Feldman)
----------------------------------------------------------------------
Message: 1
Date: Thu, 19 Jan 2006 21:36:54 -0600
From: "Hofecker, Jennifer L"
Subject: RE: [Histonet] Cat Hematoxylin
To: "histonet"
Message-ID:
<898D946569A27444B65667A49C074052852B5F@mailbe06.mc.vanderbilt.edu>
Content-Type: text/plain; charset="iso-8859-1"
I may be remembering this incorrectly, but isn't the CAT hematoxylin
named after the two wonderful men who developed it? Chuck Churukian and
David Tacha? Yes, sold by biocare... I don't remember what the
difference is but I would imagine that somebody from Biocare Medical
could help, or you could contact Charles or David...
Joe, aren't you a little afraid of what might happen to us with out
those fumes : )
Have a great weekend,
Jennifer
Jennifer Hofecker, HT (ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
(615) 343-0083
(615) 343-7089 fax
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito
Sent: Thu 1/19/2006 6:51 PM
To: Angela Bitting; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cat Hematoxylin
I think Biocare Medical sells CAT hematoxylin. It's an abbreviation for
something, I forget. Then again, I've been forgetting a lot of things
lately. What were talking about? Oh, yeah, stop sniffing the xylene. Has
to
be the xylene.
Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: "Angela Bitting"
To:
Sent: Thursday, January 19, 2006 2:38 PM
Subject: [Histonet] Cat Hematoxylin
I'm looking at a double-staining protocol that uses Cat Hematoxylin.
Does
anyone know what Cat Hematoxylin is? I don't work in research, so maybe
this
is something new or more widely utilized in a research setting. I have a
feeling that I'm going to feel really stupid when I hear the answer, but
I
can't let this go. I need to know. Thanks, in advance, for enlightening
me.
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------------------------------
Message: 2
Date: Thu, 19 Jan 2006 21:30:17 -0800
From: "Mike Cupello"
Subject: [Histonet] Need Some Heat!
To:
Message-ID: <001d01c61d82$994863d0$6401a8c0@HOME>
Content-Type: text/plain; charset="iso-8859-1"
Hi to all of those out in Histoland...
I was hoping to get some help. I have an employee who is suffering from
tendonitis, and she feels that scraping blocks before trimming is adding
to her injury. I know that there is some sort of device "out there"
that uses heat to melt the excess wax from the outside of the block so
that it will fit into the microtome chuck. Does anyone know of a type
of heat block or hot knife that could accomplish this? I am looking for
something that will collect the melted wax so that we don't have a huge
mess to clean up after a day's work.
Thanks for your time,
Mike Cupello.
------------------------------
Message: 3
Date: Thu, 19 Jan 2006 20:45:14 -0800 (GMT-08:00)
From: Mark Adam Tarango
Subject: [Histonet] marker of endothelial differentiation in vitro
To: histonet@lists.utsouthwestern.edu, DKAEK@coloplast.com
Message-ID:
<10756775.1137732314465.JavaMail.root@elwamui-karabash.atl.sa.earthlink.
net>
Content-Type: text/plain; charset=us-ascii
okay, for endothelial cells in vitro try using CD31. I know nothing
about eosin, but CD31 definately is specific for endothelial cells.
Mark Tarango
Date: Mon, 16 Jan 2006 11:05:28 +0100
From: Annette Ekblond
Subject: [Histonet] marker of endothelial differentiation in vitro
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Dear subscribers
-does anyone know of an endothelial marker specific to endothelial cells
that have differentiated into tube-like
structures (in vitro) - and not proliferating endothelial cultures??
Kind regards
Annette
CR
Humlebaek
Denmark
------------------------------
Message: 4
Date: Thu, 19 Jan 2006 23:40:19 -0800
From: mason dean
Subject: [Histonet] please remove me, thanks!
To: histonet@lists.utsouthwestern.edu
Message-ID: <469494EB-9102-4DA6-A797-02496F9CAEA9@uci.edu>
Content-Type: text/plain
------------------------------
Message: 5
Date: Fri, 20 Jan 2006 02:48:02 -0500
From: John Kiernan
Subject: Re: [Histonet] acetylcholinesterase staining
To: Angela Bitting
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <43D095B2.75BDD11F@uwo.ca>
Content-Type: text/plain; charset=us-ascii
The dire warning following your enquiry is
discouraging! Nevertheless, I make bold to reply.
I have worked in the esterase histochemistry
field, especially choline esterases, since the
early 1960s, when most of the principles had been
established.
Which method is failing to work for you half of
the time? These methods are extremely reliable if
you go by the book (any book). Complete substrate
solutions cannot be kept and used next day; that's
a rule that applies to just about all enzyme
activity histochemistry.
John Kiernan
Anatomy, UWO
London, Canada
-----------------------
Angela Bitting wrote:
>
> Our track record here with our acetylcholinesterase stain is about
50/50. We make up all the solutions fresh on Monday and it works fine,
by Tuesday ...nothing. Can anyone send me a good reliable
Acetylcholinesterase staining method? Maybe someone elses will work
better for us.
>
> IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged.
It is intended solely for the addressee. Access to this message by
anyone else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any
hard copies you may have created and notify me immediately by replying
to this email. Thank you.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 20 Jan 2006 02:48:57 -0500
From: John Kiernan
Subject: Re: [Histonet] Counter stain
To: bjdewe@aol.com
Cc: Histonet@lists.utsouthwestern.edu
Message-ID: <43D095E9.BB2F937B@uwo.ca>
Content-Type: text/plain; charset=us-ascii
The best contrasting colour for DAB-brown is
green.
Ethyl green stains nuclei, so avoid it.
(Methyl green has not been made for 25+ years.
Dyes sold as "methyl green" should be ethyl green,
which is better. Check with the vendor, because
real methyl green won't perform properly without
laborious purification by you in your lab.)
For counterstaining cytoplasm but not nuclei, try
fast green FCF. Instructions can be found in any
textbook published since about 1940. Light green
SF (pre-1940) acts similarly, but fades after a
few years.
John Kiernan
Anatomy, UWO
London, Canada
____________________________________________
bjdewe@aol.com wrote:
>
> I am doing a peroxidase DAB stain for an antigen that is present only
in small quantities in the nucleus. I am doing it on rat bone. If I
counterstain with hematoxylin it covers the DAB stain. What else can I
use that will only stain the cytoplasm and not the nucleus. i thought of
methyl green but it too is supposed to stain the nucleus. I thought of
neutral red but it won't look so good with the brown. Suggestions?
>
> Cheers,
> Lorie
>
> *******************************************************************
> Confidentiality Notice: This e-mail message, including any
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------------------------------
Message: 7
Date: Fri, 20 Jan 2006 02:50:01 -0500
From: John Kiernan
Subject: Re: [Histonet] Invertebrates in Sediment
To: BennettW@pac.dfo-mpo.gc.ca
Cc: Histonet
Message-ID: <43D09629.3D8C3B2B@uwo.ca>
Content-Type: text/plain; charset=us-ascii
Rose Bengal B is an anionic, lipophilic xanthene
dye, related to the eosins and erythrosins. One of
its documented uses is staining foraminifera in
marine sediments. Get your boss to send you to the
library to check out "Conn's Biological Stains"
and Clark's "Staining Procedures" for references
to the original methods. If it's a specialized
application, go to the references cited in these
books.
John Kiernan
London, Canada.
______________________________________________________
BennettW@pac.dfo-mpo.gc.ca wrote:
>
> Hello Histonetters,
>
> Thanks in advance for spending any time with this query! I have a
> researcher that has collected sediment samples, preserved with NBF,
and
> wants to stain any invertebrates in the sediment so that they can be
> enumerated and identified. The researcher wants to use rose bengal
but
> doesn't know at what concentration and neither do I. Can anyone help?
>
> Thanks
> Bill Bennett
> Histologist
> Fisheries and Oceans Canada
> Pacific Biological Station
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Fri, 20 Jan 2006 02:51:16 -0500
From: John Kiernan
Subject: Re: [Histonet] air drying-white sections??
To: Steven Coakley
Cc: Histonet@lists.utsouthwestern.edu
Message-ID: <43D09674.D41BE103@uwo.ca>
Content-Type: text/plain; charset=us-ascii
Dear Steven Coakley,
Please explain how you "air dry my sections before
drying them".
Also, describe exactly what you do with the
kimwipe (assuming that a kimwipe is a small paper
hanky).
If you're working with paraffin sections, it's
important to let all the water drain off the
slides (vertically) before letting the temperature
rise to anywhere near the softening point of the
wax.
Histology waxes with 58C "melting points" soften
at about 45C. If you use a hotplate, it gets
uncomfortably hot for an applied hand in 10-20
seconds at 45C. That's the maximum allowable
temperature before making a decision to melt the
wax.
The film of water between the ribbon and the glass
must be all gone before you even think about
melting the wax. This advice is in every textbook
of histotechnology (microtechnique) published
since about 1880, and it has appeared often in
Histonet for ?10 years.
The archives at http://histosearch.com are full
of wisdom in this field.
John Kiernan
Anatomy, UWO
London, Canada
____________________
Steven Coakley wrote:
>
> I've noticed that when I air dry my sections before drying them some
of them are turning a chauky white. Might that indicate anything
important. I really have not noticed this before in other places I
work. I wipe off as much excess water with a kimwipe prior to letting
the sections air dry vertically. Any thoughts.
>
> Steve
===========
------------------------------
Message: 9
Date: Fri, 20 Jan 2006 08:50:37 +0000
From: gillian.2.brown@gsk.com
Subject: Re: [Histonet] Need Some Heat!
To: "Mike Cupello"
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=us-ascii
Hi Mike,
we use a cheap domestic iron (set on delicates), standing in a shallow
collecting dish.
Gill Brown
GlaxoSmithKline Medicines Research Centre, UK
"Mike Cupello"
Sent by: histonet-bounces@lists.utsouthwestern.edu
20-Jan-2006 05:30
To
histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] Need Some Heat!
Hi to all of those out in Histoland...
I was hoping to get some help. I have an employee who is suffering from
tendonitis, and she feels that scraping blocks before trimming is adding
to her injury. I know that there is some sort of device "out there"
that
uses heat to melt the excess wax from the outside of the block so that
it
will fit into the microtome chuck. Does anyone know of a type of heat
block or hot knife that could accomplish this? I am looking for
something
that will collect the melted wax so that we don't have a huge mess to
clean up after a day's work.
Thanks for your time,
Mike Cupello.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Fri, 20 Jan 2006 08:22:45 -0500
From: "Linda Blazek"
Subject: [Histonet] job classification
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Does anyone have a dual classification of LIS manager and bench tech?
If so is the salary higher than just a bench tech?
Thanks
Linda Blazek, HT (ASCP)
Department of Pathology
Children's Medical Center
Dayton, Ohio 45404
(937) 641-3358
fax (937)641-5482
blazekl@childrensdayton.org
------------------------------
Message: 11
Date: Fri, 20 Jan 2006 09:09:24 -0500
From: "Connolly, Brett M"
Subject: [Histonet] Lot to lot antibody validation
To: histonet@lists.utsouthwestern.edu
Message-ID:
<355C35514FEAC9458F75947F5270974D67CCA5@usctmx1103.merck.com>
Content-Type: text/plain
I would like to hear what procedures people are using to validate
different
lots of antibodies, including # of specimens you might use for the
validation.
Thanks, Brett
Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
WP-44K
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly@merck.com
------------------------------------------------------------------------
------
Notice: This e-mail message, together with any attachments, contains
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outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD
and in Japan, as Banyu) that may be confidential, proprietary
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of the individual or entity named on this message. If you are not the
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------------------------------
Message: 12
Date: Fri, 20 Jan 2006 08:43:52 -0600
From: "Orr, Rebecca"
Subject: [Histonet] slide storage feedback
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Thanks everyone for your response!
I got a lot of advice on long term slide storage sent to me privately,
so just to share, I would say the general consensus was ultra low temps
wrapped in foil along with a desiccant.
Thanks again to the wonderful Mr. Tim Morken for sharing this article on
the TMA storage and the paraffin "dip". The technique was what the
Pathologist was looking for and what we will be using in our project.
The Histonet is such a valuable tool, and I appreciate the support and
help I have gotten from peers as well as vendors.
Have the best weekend possible! I'll be spending my Saturday shoveling
snow.
Becky
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
------------------------------
Message: 21
Date: Thu, 19 Jan 2006 15:50:02 -0600
WS
------------------------------
Message: 13
Date: Fri, 20 Jan 2006 09:45:41 -0500
From: "Amy Porter"
Subject: Re: [Histonet] Lot to lot antibody validation
To: "Connolly, Brett M" ,
Message-ID: <000301c61dd0$2fd29210$8e7a0923@HistoJJ>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
We use a known postive control and run a system negative (no primary)
with
each new lot of primary that is received. We only run it one time maybe
at
the dilution for the old lot then slightly higher and slightly lower
dilution with the already known pretreatment. Slides are then reviewed
and
the acceptable dilution for the new lot is recorded on an "Antibody
Titration Log Sheet" signed off on by the pathologist or researcher to
whom
the work is being provided for. Hope this helps out...
----- Original Message -----
From: "Connolly, Brett M"
To:
Sent: Friday, January 20, 2006 9:09 AM
Subject: [Histonet] Lot to lot antibody validation
>I would like to hear what procedures people are using to validate
different
> lots of antibodies, including # of specimens you might use for the
> validation.
>
> Thanks, Brett
>
> Brett M. Connolly, Ph.D.
> Merck & Co., Inc.
> MRL, Imaging Research
> WP-44K
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-993-6803
> e-mail. brett_connolly@merck.com
>
>
>
>
------------------------------------------------------------------------
------
> Notice: This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New
> Jersey, USA 08889), and/or its affiliates (which may be known outside
the
> United States as Merck Frosst, Merck Sharp & Dohme or MSD and in
Japan, as
> Banyu) that may be confidential, proprietary copyrighted and/or
legally
> privileged. It is intended solely for the use of the individual or
entity
> named on this message. If you are not the intended recipient, and
have
> received this message in error, please notify us immediately by reply
> e-mail and then delete it from your system.
>
------------------------------------------------------------------------
------
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 14
Date: Fri, 20 Jan 2006 08:53:55 -0600
From: "Orr, Rebecca"
Subject: [Histonet] Cat Hematoxylin
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Angela,
I use CAT hematoxylin as the counterstain on all of my IHC single and
doublestains.
It is a special formulation that does not need filtering.
As I recall, the CAT denotes the initials of the scientists who
formulated it,
Cherukian, Allison, Tacha. I buy mine from Biocare Medical.
www.biocare.net
Let me know how your double stains are working. We use a few routinely,
here.
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
------------------------------
Message: 15
Date: Fri, 20 Jan 2006 08:57:40 -0600
From: "Rey, Jean-Philippe"
Subject: RE: [Histonet] Need Some Heat!
To: "Mike Cupello" ,
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Mike,
Thermo is offering Shandon PARA trimmer, a "paraffin melter" that we are
currently using in the lab to trim off the excess of paraffin from our
block. It is really efficient and honestly less messy that the wax chips
scattering everywhere. We usually use plastic cup to collect melting
wax.
Have a good one.
JP REY
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mike
Cupello
Sent: Thursday, January 19, 2006 11:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Need Some Heat!
Hi to all of those out in Histoland...
I was hoping to get some help. I have an employee who is suffering from
tendonitis, and she feels that scraping blocks before trimming is adding
to her injury. I know that there is some sort of device "out there"
that uses heat to melt the excess wax from the outside of the block so
that it will fit into the microtome chuck. Does anyone know of a type
of heat block or hot knife that could accomplish this? I am looking for
something that will collect the melted wax so that we don't have a huge
mess to clean up after a day's work.
Thanks for your time,
Mike Cupello.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Fri, 20 Jan 2006 09:07:10 -0600
From: "Ford Royer"
Subject: RE: [Histonet] Need Some Heat!
To:
Message-ID: <003001c61dd3$307affb0$6f01a80a@fords>
Content-Type: text/plain; charset="us-ascii"
Ditto...
Check out the Thermo Shandon "Para Trimmer", SKU (catalog number):
B3120205
Web page:
~ Ford
Ford M. Royer, MT(ASCP)
Sales Manager, Histology Product Division
Minnesota Medical Specialists, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
763-542-8725 phone
888-790-9686 toll free
763-546-4830 fax
email
web
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rey,
Jean-Philippe
Sent: Friday, January 20, 2006 8:58 AM
To: Mike Cupello; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Need Some Heat!
Mike,
Thermo is offering Shandon PARA trimmer, a "paraffin melter" that we are
currently using in the lab to trim off the excess of paraffin from our
block. It is really efficient and honestly less messy that the wax chips
scattering everywhere. We usually use plastic cup to collect melting
wax.
Have a good one.
JP REY
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mike
Cupello
Sent: Thursday, January 19, 2006 11:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Need Some Heat!
Hi to all of those out in Histoland...
I was hoping to get some help. I have an employee who is suffering from
tendonitis, and she feels that scraping blocks before trimming is adding
to
her injury. I know that there is some sort of device "out there" that
uses
heat to melt the excess wax from the outside of the block so that it
will
fit into the microtome chuck. Does anyone know of a type of heat block
or
hot knife that could accomplish this? I am looking for something that
will
collect the melted wax so that we don't have a huge mess to clean up
after a
day's work.
Thanks for your time,
Mike Cupello.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Fri, 20 Jan 2006 08:37:16 -0700
From: Gayle Callis
Subject: [Histonet] Re: IHC slides and sections
To: bjdewe@aol.com, Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060120083318.01b62688@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Erie's new EXEL slides (I may have spelled that incorrectly), they also
make slides for microarray work - give them a call.
Newcomers Supply has slides they maintain are more than just Plus charge
-
they have a website.
Superfrost Gold although these are touted for use with frozen sections
and
not necessarily paraffin sections, but they are as described, pricey
little
gold nuggets. Some have used these for paraffin and thought it improved
holding onto section. You can find these comments in Histonet Archives.
At 01:04 PM 1/19/2006, you wrote:
>
> So what slides are the most adhesive? I only knew about the
Superfrost +
> slides. Are there more brands??
>
>Lorie
>
>*******************************************************************
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is
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>
>-----Original Message-----
>From: Osborn, Sharon
>To: 'histonet@lists.utsouthwestern.edu'
>Sent: Thu, 19 Jan 2006 13:36:54 -0500
>Subject: [Histonet] IHC slides and sections
>
>
>Thanks a bunch for all the wonderful suggestions. We tried several of
the
>suggestions and sections still came off. The charged slides were still
'in
>date'. We switched to a different slide and the sections remaned on.
The
>charged slides have different "stickiness" depending upon the vendor.
In
>the past we have used some slides that were 4-5 years old and the
>stickiness was still present-excellent charge retention I guess.
However,
>we now have solved the problem with a stickier slide on the charge.
Your
>suggestions are helpful for our use overall and several are being
implement
>as a regular protocol.
>
>Have a great weekend! And, again THANKS!
>
>Sharon Osborn
>DNAX, SP BioPharma
>Palo Alto, CA
>650-496-6539
>
>
>*********************************************************************
>This message and any attachments are solely for the intended recipient.
If
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>_______________________________________________
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>_______________________________________________
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>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
------------------------------
Message: 18
Date: Fri, 20 Jan 2006 08:48:55 -0700
From: Gayle Callis
Subject: Re: [Histonet] Cat Hematoxylin
To: "Joe Nocito" ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060120084359.01b6b738@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
It has something to do with a "Masterpiece" i.e. they (BIocare) also
have
something using Degas in the name.
Trying to figure out who CAT is that produced masterpiece paintings,
sculpture or whatever??? Want to bet it is some kind of progressive
hematoxylin like Mayers, Gill recipes. Maybe they will step forward and
explain the "artsy" nomenclature.
At 05:51 PM 1/19/2006, you wrote:
>I think Biocare Medical sells CAT hematoxylin. It's an abbreviation for
>something, I forget. Then again, I've been forgetting a lot of things
>lately. What were talking about? Oh, yeah, stop sniffing the xylene.
Has
>to be the xylene.
>
>Joe Nocito BS, HT(ASCP)QIHC
>Histology Manager
>Pathology Reference Lab
>San Antonio, TX
>
>----- Original Message ----- From: "Angela Bitting"
>To:
>Sent: Thursday, January 19, 2006 2:38 PM
>Subject: [Histonet] Cat Hematoxylin
>
>
>I'm looking at a double-staining protocol that uses Cat Hematoxylin.
Does
>anyone know what Cat Hematoxylin is? I don't work in research, so maybe
>this is something new or more widely utilized in a research setting. I
>have a feeling that I'm going to feel really stupid when I hear the
>answer, but I can't let this go. I need to know. Thanks, in advance,
for
>enlightening me.
>
>
>
>IMPORTANT WARNING: The information in this message (and the documents
>attached to it, if any) is confidential and may be legally privileged.
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>is intended solely for the addressee. Access to this message by anyone
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>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 19
Date: Fri, 20 Jan 2006 09:00:27 -0700
From: Gayle Callis
Subject: Re: [Histonet] Need Some Heat!
To: "Mike Cupello" ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20060120085111.01b69d18@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Mike,
I have a young lady in the same boat, but her employer is doing nothing
about it although the following device has been recommended. She
resorted
to embedding so the paraffin amount is just enough to not create this
problem - a tedious way to do it, but it does work - however others
embedding are not so careful.
Go to Thermo Electron and look for their heated paraffin trimmer with
slanted, heated surface and paraffin capture tray. (ParaTrimmer, not
sure
of the name) - it is a bit pricey, but well worth the money and I would
perosnally love to have one in our lab.
You employee's problem may stem from more than just scraping blocks, and
you need to look at other ergonomics involved with her microtomy,
embedding
,etc etc. Does she operate the microtome in an ergonomic manner, have
an
ergonomic chair, decent setup for reach, forceps that help her out, all
those good things. Also what she does outside the lab may be a factor
as
the young lady here does some other physical activities (lots of cell
phone
dialing is one using her thumb to punch in the numbers) that contributes
to
her problems in the lab. Hopefully Jan Minshew, an expert on ergonomics
in
the histology laboratory can help you here too.
At 10:30 PM 1/19/2006, you wrote:
>Hi to all of those out in Histoland...
>
>I was hoping to get some help. I have an employee who is suffering
from
>tendonitis, and she feels that scraping blocks before trimming is
adding
>to her injury. I know that there is some sort of device "out there"
that
>uses heat to melt the excess wax from the outside of the block so that
it
>will fit into the microtome chuck. Does anyone know of a type of heat
>block or hot knife that could accomplish this? I am looking for
something
>that will collect the melted wax so that we don't have a huge mess to
>clean up after a day's work.
>
>Thanks for your time,
>
>Mike Cupello.
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 20
Date: Fri, 20 Jan 2006 08:10:48 -0800
From: "Robyn Vazquez"
Subject: Re: [Histonet] Need Some Heat!
To: histonet@lists.utsouthwestern.edu, mike_cup@telus.net
Message-ID:
Content-Type: text/plain; charset=us-ascii
Mike,
Why don't you appoint someone to scrap her blocks or all of them, until
her tendonitis heals? We used to have a non-tech do this task and it
would save us time. Tendonitis is only temporary generally.
Robyn
OHSU
------------------------------
Message: 21
Date: Fri, 20 Jan 2006 11:13:57 -0500
From: "Bartlett, Jeanine"
Subject: RE: [Histonet] Need Some Heat!
To: "Gayle Callis" , "Mike Cupello"
,
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
The part number is B3120205 and it is called a Para Trimmer...they run
around $500.00. But honestly, there is no need for scraping blocks if
you embed carefully. I haven't had to scrape a block (except when I
accidentally tipped one over on the embedding center cooling tray) in
years.
Jeanine Bartlett, HT(ASCP)
(404) 639-3590
jeanine.bartlett@cdc.hhs.gov
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Friday, January 20, 2006 11:00 AM
To: Mike Cupello; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need Some Heat!
Mike,
I have a young lady in the same boat, but her employer is doing nothing
about it although the following device has been recommended. She
resorted to embedding so the paraffin amount is just enough to not
create this problem - a tedious way to do it, but it does work - however
others embedding are not so careful.
Go to Thermo Electron and look for their heated paraffin trimmer with
slanted, heated surface and paraffin capture tray. (ParaTrimmer, not
sure of the name) - it is a bit pricey, but well worth the money and I
would perosnally love to have one in our lab.
You employee's problem may stem from more than just scraping blocks, and
you need to look at other ergonomics involved with her microtomy,
embedding ,etc etc. Does she operate the microtome in an ergonomic
manner, have an ergonomic chair, decent setup for reach, forceps that
help her out, all those good things. Also what she does outside the lab
may be a factor as the young lady here does some other physical
activities (lots of cell phone dialing is one using her thumb to punch
in the numbers) that contributes to her problems in the lab. Hopefully
Jan Minshew, an expert on ergonomics in the histology laboratory can
help you here too.
At 10:30 PM 1/19/2006, you wrote:
>Hi to all of those out in Histoland...
>
>I was hoping to get some help. I have an employee who is suffering
>from tendonitis, and she feels that scraping blocks before trimming is
>adding to her injury. I know that there is some sort of device "out
>there" that uses heat to melt the excess wax from the outside of the
>block so that it will fit into the microtome chuck. Does anyone know
>of a type of heat block or hot knife that could accomplish this? I am
>looking for something that will collect the melted wax so that we don't
>have a huge mess to clean up after a day's work.
>
>Thanks for your time,
>
>Mike Cupello.
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Fri, 20 Jan 2006 11:19:47 -0500
From: Ada Feldman
Subject: Re: [Histonet] Z fix
To: "Molinari, Betsy"
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <4FE5AD65-F73E-4DE6-BBD4-5043CD670FA1@anatechltdusa.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Dear Betsy,
Store the heart tissue in Z-FIX. Our in-house data show tissues
stored in Z-FIX for 30 days show no deleterious effects on staining
including immunohistochemistry.
Sincerely,
Ada T. Feldman, MS, HT/HTL(ASCP)
Director of Manufacturing & Technical Services
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49015
Phone: 800.262.8324
Fax: 269.964.8084
email: adafeldman@anatechltdusa.com
website: www.anatechltdusa.com
On Jan 19, 2006, at 1:49 PM, Molinari, Betsy wrote:
> Hi,
>
> I have some mouse hearts in Z Fix. I am going to be out of town for
> the
> next week, can they stay in Z Fix or should I transfer them to 70%?
>
> Thanks.
>
>
>
> Betsy Molinari HT (ASCP)
>
> Texas Heart Institute
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave.
>
> Houston,TX 77030
>
> 832-355-6524
>
> 832-355-6812 (fax)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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