Kristen
I am not trying to justify any response that you received but I think
that I understand why the response was phrased in that particular
manner. 
Many of the questions that arrive at Histonet are very specific, and
generally get a lot of responses. 
The broader questions are often difficult to answer (and in general I
personally do not respond to these) and often receive few responses.  
It is usually not possible to determine the level of knowledge of the
individual who is asking the question and there may be a wide variety of
answers. 
Here on Histonet we are often between a rock and a hard place. I believe
that it is a natural tendency nowadays to pose the question in the
shortest way possible on the assumption that nobody wishes to spend
their time to read a long discourse. This unfortunately often results in
a communication that is open to a wide variety of interpretations. One
interpretation is that the individual posing the question wants to be
informed about all aspects of a particular technique from anesthetizing
an animal to interpreting the final result, rather than carrying out the
initial groundwork themselves. There is usually no way to judge if this
is true from the original communications.
This is the era of rapid fire information but unfortunately the
recipients do not always have the same background or experiences to view
that information in the same light. 
In many cases I feel that in response to complex questions, individuals
with the appropriate expertise should provide their telephone number so
that a dialogue can be set up. While Histonet is very useful, a one on
one dialogue generally will provide the most relevant information.
I hope that you will continue to ask questions on Histonet.
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen
Reynolds
Sent: Thursday, January 20, 2005 9:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] perfusion suggestions

Wow, I can't believe how rude of a response I got.  I
thought this was for helpful comments.  I know how to
perfuse for light microscopy and EM.  I just thought
the fixative combination for wanting to do both on the
same tissue might be out there.  I guess I won't be
asking histonet anything anymore.

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