I blame it on all those years of inhaling xylene fumes before the phrase
"Health and Safety" was invented.

Demyelinatedly yours

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

-----Original Message-----
From: Stapf, Ross [mailto:RossS@BaylorHealth.edu]
Sent: 20 January 2004 19:53
To: Nick Kirk; Histonet
Subject: RE: [Histonet] C4d antibody


I made the same mistake for the first month I was here.

You should have seen the look on my immuno techs' faces when after they
asked me to find a new source for C4D, I handed them a whole bunch of
CD4 spec sheets.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS@baylorhealth.edu



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick
Kirk
Sent: Tuesday, January 20, 2004 1:43 PM
To: Histonet
Subject: RE: [Histonet] C4d antibody


Oops, must need my eyes testing, just read this again and noticed it is
an enquiry for C4d not CD4. Ignore the previous mumblings of an addled
person.

Nick Kirk

-----Original Message-----
From: Nick Kirk [mailto:nick.kirk3@btopenworld.com]
Sent: 20 January 2004 19:41
To: Histonet
Subject: RE: [Histonet] C4d antibody


We use Novocastra's CD4 on FFPE sections using Vector's Antigen
Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection
kit which uses DAB. We use our routine method and have few problems
getting it to work. The biggest problem is the availability of good
quality tonsil control material, that hasn't been fixing for years in
the cupboard.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Featherstone, Annette
Sent: 20 January 2004 18:41
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody


Hi

Anyone out there using C4d antibody? Have to work it up and want to know
if there are any tips to make this work as they haven't been able to get
it to work right. Manufacturer recommends Alk Phos, but I was hoping to
get it to work with DAB. Any suggestions? Thanks Annette Featherstone
HT/MLT




-----Original Message-----
From: Instrumedics [mailto:info@instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover


One of the advantages of the Cryo-Vac-Away vacuum system for the
cryostat is that the debris generated during the trimming step is
suctioned away as  it is generated at the blockface. This substantially
reduces the chances that any sections will cling to the underside of the
knife which can then can carryover to the following case.

Bernice
schiller@instrumedics.com



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE:
This email transmission and any documents, files,
or previous e-mail messages attached to it are
confidential and intended solely for the use of the
individual or entity to whom they are addressed.
If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient,
you are hereby notified that any further review,
disclosure, copying, dissemination, distribution, or
use of any of the information contained in or attached
to this e-mail transmission is strictly prohibited.
If you have received this message in error, please
notify the sender immediately by e-mail, discard
any paper copies, and delete all electronic files
of the message. If you are unable to contact the
sender or you are not sure as to whether you
are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or
call (716) 859-7777.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet