What exactly are you referring to for "tissue freezing medium". Do you mean
the OCT? If you do, the OCT should not touch the frozen muscle that has been
snappped frozen in liquid nitrogen, we use just a small amount on a chuck
and put one frozen end of the muscle on it. Just enought to stick the muscle
to the chuck. We place the chuck in crushed dry ice and this works very
well. OCT freezes slow and will cause freeze artifact in your muscle as it
will work to thaw it.
Annette Featherstone HT




-----Original Message-----
From: Anacko, Nancy L. [mailto:NAnacko@Lifespan.org]
Sent: Friday, January 17, 2003 09:38
To: 'Server, Histonet'
Subject: frozen muscle


Hi Histonetters :)
A question to all who work with frozen muscle and do enzyme staining....does
the "tissue freezing medium" interfere with the enzyme staining ?? Please
help :)
Thanks,
Nancy

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