I don't know the stains mentioned, but can you do TUNEL in 0.5 ml volume
of fluid with free floating sections?  We offer a mini tray (the
Histodipper) for reactions on free floating sections with expensive
reagents.  See the following link:  

http://www.myneurolab.com/mnl/mnlsite/ViewProduct.asp?idproduct=464101&c
atdesc=Histology+Equipment&subcatdesc=Tissue+Staining&idsubcategory=192


Does this help?

Cordially,

Charles W.  Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0377
cwscouten@myneurolab.com
www.myneurolab.com


-----Original Message-----
From: Matt McElwee [mailto:MCELWEE@surgery.wisc.edu] 
Sent: Friday, January 24, 2003 10:28 AM
To: histonet@pathology.swmed.edu
Subject: Apoptosis

Hello Histonetters.
I'm trying to find a cost-effective way to measure apoptosis in free
floating mouse brain sections.  I know that TUNEL is the gold standard
for apoptosis, but, from what I've seen, it would be prohibitively
expensive to do this in the relatively large volume required for free
floating sections.  I was thinking it might be cheaper to do IHC for
cleaved caspase-3 where I could do an extended incubation in highly
diluted antibody.  Does anyone know of a good antibody to cleaved
caspase-3, or any other protein specific to apoptosis?  Any help would
be greatly appreciated.  Thank you.
Matt   

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648
mcelwee@surgery.wisc.edu