We should not forget the lipid-dissolving properties of dehydrating agents
(ethanols). A colleague did a little study more than a few years ago and
measured how much lipid was extracted in the ascending ethanol series. I don't
remember the numbers but it was very substantial.

Geoff

"Charles.Embrey" wrote:

> ORO for fat staining only realistically works on frozen tissue as fat
> dissolves in the clearing agent during processing.  Basic histotech 101 and
> a decent HT exam question.
> Chuck
>
> -----Original Message-----
> From: dgaupp@tulane.edu [mailto:dgaupp@tulane.edu]
> Sent: Monday, January 28, 2002 6:21 PM
> To: histonet@pathology.swmed.edu
> Subject: Oil Red O
>
> Hello Histonetters,
>
> Does anyone have a protocol for staining ORO on paraffin sections?
>
> Thanks,
>
> Dina

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************