Thank you for everyone for their help.  I am in the process of evaluating
your suggestions.

Your list server is a tremendous source of help and info.

Yours sincerely,

Judit Zubovits

-----Original Message-----
From: Huib Croes [mailto:h.croes@mailbox.kun.nl]
Sent: Friday, January 11, 2002 12:26 PM
To: Zubovits, Dr. Judit
Subject: Re: vital stain


Dear Dr. Judit Zubovits

Maybe the procedure in this article can help you. I've never tried it myself
though.

Jona VAlaitis et all.
Detection of early acute myocardial infarction by gomori trichrome aniline
blue stain.
The journal of histotechnology/ Vol. 17 No.2/june 1994

A time ago Dr. Geert Smits wrote an article on the effect of high energy
shock waves on mouse skeletal muscle as part of his thesis. It was in press
in ultrasound in medicine & biology 1992.

Effects of high energy shock waves in mouse skeletal muscle ..........

Kind regards

Huib Croes
Dept. Cell Biology  163
Cath. Univ. of Nijmegen
POBOX 9101
6500 HB Nijmegen
h.croes@mailbox.kun.nl

----- Original Message -----
From: Zubovits, Dr. Judit 
To: 
Sent: Wednesday, January 09, 2002 4:19 PM
Subject: vital stain


> Hello Histonet-ters;
>
> I wonder if you could help me with the following:
>
> I am looking at "muscle biopsies" from volunteers (mice) who have received
> focused ultrasound treatment.  Of course enough excess heat would cook any
> tissue, but I we are trying to detect much more subtle changes/damage
> produced by a lot less heat.
>
> I know TTC has been used on hearts to detect ischemic necrosis associated
> with myocardial infarction, but as I understand it, you need to soak the
> tissue *before* paraffin embedding, and TTC is intended to show areas of
> necrosis at the gross, not microscopic level.
>
> Is there anything out there for the microscopic level that would highlight
> areas of damage?
>
> Thanks for all your help.
>
> Judit Zubovits
>