Aneeta,

When we did this "in house" we used Novocastra's, cat. # NCL-CD4-1F6, at
1:25.  HIER was in 1mM EDTA, pH 8, in Biocare's DeCloaking chamber for 2.5".
The primary antibody incubated for 28" and was amplified. Staining took
place on Ventana automated immunostainers.  

Due to low volume requests for this antibody we now send these out to a
reference lab.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Aneeta Patel [mailto:apatel@usuhs.mil]
Sent: Tuesday, January 08, 2002 11:51 AM
To: Histonet@pathology.swmed.edu
Subject: cd4


Hi
Does anybody have a method/source of antibody for CD4 on human paraffin
embedded tissues.
thanks