On Mon, 29 Jan 2001, Terrett, Barb wrote:

> Is anyone using CD25 (ours is from Novocastra) , and if so how is it working
> for you? We have had some problems (using it on FFPE) . We are microwaving
> it in citrate buffer pH 6.0 and staining with the envision kit. I was
> gettingtoo much background with my usual link and label system, even at a
> high titre.
> Thanks for your help
> b
> 
Hello Barb,
I too was having problems with CD25 but got it to work as follows:
pretreat with Dako's TRS (not the high pH one)
1:50 dilution and 60 minute incubation using a tonsil as positive control.
Good Luck.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA