VSV-G

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From:Emma Carter <E.Carter@OxfordBioMedica.co.uk> (by way of histonet)
To:histonet@histosearch.com
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(posted for a colleague)

Dear All,

We are trying to detect the vesicular stomatitis virus glycoprotein in
western blotting.  We are using the commercially available, P5D4,
monoclonal antibody to the cytoplasmic tail of VSV-G.  Unfortunately
this recognises another protein of similar size to the VSV-G in our cell
line TE671.  Does anybody know of another antibody or of a source for a
polyclonal serum against VSV-G which we could use?? Or have any tips to
prevent this happening?

F.M.Ellard




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