If the the efficiency of the vector is high and if the cells are producing the construct then you should be able to see the GFP with no problem in frozen  and paraffin tissue.

Angeline Martin-Studdard wrote:

> Hello All,
>
> I will soon be delving into the visualization of GFP positive cells that have been transplanted into normal mice.  I have seen a few blips on the histonet regarding problems with this protein.  I will be working with brain tissue sections and will try both frozen and paraffin embedded.  I'd like to know if anyone else has experience with this.  Can the fluorenscence from the protein still be seen after processing of the tissue or does it require detection and/or amplification?
>
> Any advice or assistance you can offer will be very helpful.
>
> thank you,
> angeline
>
> angeline martin
> cell biology and anatomy
> medical college of georgia
> augusta, ga
> amartin@mail.mcg.edu