we tried both the polyklonal CD3 from DAko and the monoklonal CD3 from
Novocastra. Both work well, but in my opinion, the monoclonal antibody
doesn't stain all the T-cells, or at least, stains fewer lymphocytes than
the DAKO polyklonal. HIER is done for 10 min in pH 6 citrate-buffer in a
preasure cooker, dedection kit is from DAKO. All material is fixed in
neutral buffered formalin for 24 hours, BM-trephines are decalcified for 24
hours (at least) in EDTA on a shaking board (that reduces the time for
complete decalcification a lot).




Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
A 1140 Wien, Oesterreich
 
Alexander.Nader@wgkk.sozvers.at <mailto:Alexander.Nader@wgkk.sozvers.at>

privat: Alexander.Nader@univie.ac.at
<mailto:Alexander.Nader@univie.ac.at>


> -----Ursprüngliche Nachricht-----
> Von: Richard Cartun [mailto:Rcartun@harthosp.org]
> Gesendet am: Donnerstag, 06. Jänner 2000 16:35
> An: Histonet
> Betreff: CD3
>
> I am interested in hearing what Histoneters are using for the
> detection of CD3+ cells in paraffin sections (pAb vs. mAb,
> digestion vs. HIER, etc.).  Thanks.
>
> R. Cartun
>
>