We use this antibody at a 1:25 dilution with EDTA antigen retrieval at the
ph of 8.0.
We use a steamer for 60 minutes for the AR but, the protocol I have
uses a microwave.    Hope this helps..    Also cut the controls fresh.
Hazel


> -----Original Message-----
> From:	Fessler, Jim [SMTP:jifess@pahosp.com]
> Sent:	Thursday, January 11, 2001 7:37 AM
> To:	'histonet@pathology.swmed.edu'
> Subject:	Alk Protein Antibody
> 
> I am trying to get the Alk Protein monoclonal antibody from Dako to work
> (with no success).  Any suggestions would be greatly appreciated. thanks