Iron stain counterstain
From: | Andrea Grantham <algranth@u.arizona.edu> |
Histonetters -
I was wondering if there was another stain (other than Nuclear Fast Red)
that could be used as a counterstain for the iron stains?
My next question is - what can be done to prevent the white precipitate
from adhering to the slides that have been in Nuclear Fast Red
(Kernechtrot)Solution? I have had this problem with several batches of the
stain - not just in this lab with these chemicals. I am sure that I am
making up the solution correctly and just to be certain I had another tech
make it up using the "recipe" from the green AFIP book (page 179). Still
the same problem.
Any advice/help would be greatly appreciated since this is driving me nuts!
Thanks!
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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