Although we don't currently use Pen-Fix, we used it for years on our
processor after 10% formalin and did not find it to affect immunos
adversely.

Marg
Supervisor, Anatomic Pathology
Eisenhower Medical Center
39-000 Bob Hope Drive
Rancho Mirage, CA 92270


-----Original Message-----
From: Donna Sitrin [mailto:dsitrin@unipathllc.com]
Sent: Friday, January 12, 2001 10:14 AM
To: histonet@pathology.swmed.edu
Subject: Penfix


Has anyone run into the following processing protocol, and do they have any
experience with immunos on tissue processed this way?

1.5 hours fixation in 10% neutral buffered formnalin,
followed by
1.5 hours in Penfix

Has anyone had IH porblems associated with Penfix?  I do immunos on outside
tissue on occasion, and have run into this type of processed tissue.  Any
help would be greatly appreciated.

Thanks in advance for any information, good or bads, that you can give me.
Donna Sitrin
UniPath, LLC