Re: hematoxylin & eosin frozen section staining

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From:"Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Date:          Fri, 26 Feb 1999 18:49:39 -0500 (EST)
From:          Arlene N Taylor <cloud39@juno.com>
Subject:       hematoxylin & eosin  frozen section staining
To:            histonet@pathology.swmed.edu


Dear Arlene,

We fix the section on the slide in Neutral Buffered Formalin for 10
secs.

Rinse in tap water, both sides of slide.
Haematoxylin (Mayer's Westmead Modification)     10 secs.
Rinse in tap water, both sides of slide.
Scott's Blueing Solution                                 10secs.
Rinse in tap water, both sides of slide.
Eosin (Eosin/Erythrosin water based)              10secs
Rinse in Absolute ethanol                               10secs
Xylene                                                     10secs
Mount.
_______________________________________________________
Hi,
I'm collecting  procedures for hematoxylin and eosin  of staining
frozen sections. Thanks in advance.

___________________________________________________________________
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Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330
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