Re: Microwave Processing
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From: | "Aidan C. Schurr" <aidan.schurr@stonebow.otago.ac.nz> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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>Hello-
>I have just joined the histonet, so I am not sure if this topic has been
>discussed recently. I was curious if anyone has been successful in doing
>microwave fixation, processing or decalcification. If so, I would like to
>know what kind of microwave you use, any protocols available and what you
>personally think about the whole idea. If you have any references to
>companies that sell microwaves that would be helpful too. We are interested
>in processing larger tissues in a faster time frame (aren't we all??!!?)
>
>Carrie Schray, HT(ASCP)
>Parke-Davis Pharmaceuticals
>Ann Arbor, Michigan
We have a standard kenwood mw oven, as well as a commercial laboratory
setup. The standard Kenwood oven (with a temperature probe) is used for
the demonstration of microwave assisted decalcification and formalin
fixation for our third year students, with good results.
For an excellent resource of all things microwave, check out:
"Microwave cookbook for microscopists", by L.P. Kok and M.E. Boon, ISBN:
90-71421-20-1
Good luck
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Aidan C. Schurr BMedLabSc
Histology Service Unit ++64 3 4797152
Pathology Department
Dunedin School of Medicine
acschurr@bigfoot.com
aidan.schurr@stonebow.otago.ac.nz
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