Ian,

I have been using antibodies that were made for us ~12 years ago which were
aliquated and stored in one of our -80 freezes and they still work
perfectly.  Don't know about storing them at -196, I would doubt that it
would hurt them but I don't think that it would extend their usfullness.

I do know that when these same antibodies were stored at -20 (some were put
in the -20 by a predecessor) they started to lose potency after about 3
years.  That was when I 'discovered' the ones stored in the -80.

-- Begin original message --
>
>       If I store antibodies at -80 or -196 in nitrogen will I extend the
> expiry date or am I just wasting my time and should stick to the
> recommended -20.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk

-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**