Urgent help needed!
for months we did immmunohistochemistry on formalin-fixed,
paraffin-embedded tissues for bcl2 with no problems. We use the
monoclonal from Dako, dilution 1:40, 5 min pressure cooking in citrate
buffer pH 6.0
And now, all over sudden, it does not work anymore, only very very faint
staining. We checked dilutions and even tried antibody "pure". Expiration
date of the lot is 2001.
Any suggestions? How do you pretreat your tissue sections for bcl2?

thanks for your help,

Heike Grabsch, Germany