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---HistoNet Server <HistoNet@Pathology.swmed.edu> wrote:
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 01:30:11 -0600
> From: "bbracing" <bbracing@silk.net>
> Subject: Re  Diastase/Amylase
>
> Many thanks from myself and my staff to all of you who answered my
query on
> Diatase/Amylase digestion of Glycogen.  We tried many of the
sugestions, using
> newly purchased products, with considerable success.  After several
small
> blind studies on different control sections, some digested with
saliva, others
> with Amylase formulated as sugested, we settled on the following.  A
 0.2%
> aquous solution of amylase (in distilled water, sigma A3176) for 20
min at
> room temp.  This gave us the convenience of use that fit our work
pattern, and
> in the blind study no one was able to detect which slides were
digested by
> what.
> So now to everyones relief -- No More Spitting!.
> Kerry Beebe
> Kelowna Gen Hospital
> Kelowna B.C. Canada
> bbracing@silk.net
>
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 01:30:34 -0600
> From: kevin gibbon <gibbowax@uniserve.com>
> Subject: Antibodies needed
>
> Hi everybody,
> does anyone have any idea where I could get antibodies for
> immunohistochemistry on paraffin sections to  Bacillus cereus and
Bacillus
> thuringiensis.
>
> Thanks
> Kevin Gibbon
> Wax-it Histology services
>
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 05:30:45 -0600
> From: "Marjorie & David Cardwell" <cardwell@connexus.net.au>
> Subject: MUSCLE HISTOCHEMISTRY - problems with ATPases?
>
> Has anyone had problems with inconsistent ATPase staining on muscles,
> especially at pH4.6?
>
> I'd be very interested to hear of people's approaches to this stain.
>
> David Cardwell
> State Neuropathology Service
> Melbourne, Australia
>
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 06:15:56 -0600
> From: "Sue.Hacker" <Sue.Hacker@bbsrc.ac.uk>
> Subject: Formic acid treatment of Scrapie Tissues
>
> Greetings Histonetters,
> Can any of you help. We are receiving Formic acid treated tissues
from
> Scrapie infected animals, and quite frankly the tissue leaves a lot
to be
> desired !
> Any suggestions on improving the quality of the sections produced.
> The tissues have been treated for 1 hour , but in some cases 1 and
half
> hours. The problems we are finding are HE's falling off slides, very
> brittle tissues, etc etc.
> Some researchers have suggested that we do not need to formic acid
treat
> scrapie tissues as they are not as hazardous as BSE and CJD tissues,
your
> views on this would be helpful.(My view is better safe than sorry! )
> Any help is much appreciated.
> Thanks.
> Sue Hacker.
> IAH. Compton.
> Newbury.Berks.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 08:00:12 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: MUSCLE HISTOCHEMISTRY - problems with ATPases?
>
> >Date: Tue, 09 Feb 1999 22:22:01 +1100
> >From: Marjorie & David Cardwell <cardwell@connexus.net.au>
> >Subject: MUSCLE HISTOCHEMISTRY - problems with ATPases?
> >To: Histonet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Has anyone had problems with inconsistent ATPase staining on muscles,
> >especially at pH4.6?
> >
> >I'd be very interested to hear of people's approaches to this stain.
> >
> >David Cardwell
> >State Neuropathology Service
> >Melbourne, Australia
> >
>
> David,
> 	Inconsistent ATPase staining, is this not normal. The most
> consistent method I have used is that by Joan Round. The technique is
> reliable for human muscle but usually needs a wee bit of tinkering
with the
> pH's for other species. The one thing I do find with all ATPase
techniques
> is that fresh sections are best. Human muscle is more tolerant but
other
> species can be unreliable at acid pH after short term storage. If
you can't
> get the paper let me know and I'll e-mail the method.
> Ian.
> Round,J.M., et al. 1980. Histochem. J. 12. 707-710.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 08:10:40 -0600
> From: "Shelly Christenson" <CHRISTEN@vet.ksu.edu>
> Subject: thank you
>
> Thanks to all that gave me info. on the Histology schools. I am
> gratefull for all of your help and advice.
> Thank You
> Shelly
>
>
> ----------------------------------------------------------------------
>
> Date: 9 Feb 1999 08:30:14 -0600
> From: oshel@terracom.net (Philip Oshel)
> Subject: Re: Dremel tool
>
> Carol & listers,
>
> Dremels are nice, but they are really hobbyist tools and are
underpowered.
> They'll do the job, but more slowly and with less control, and have
shorter
> lifetimes. Check your local college's art department metals program,
or a
> local metalsmith/jeweler for recommendations for flex-shaft machines.
> According to my local metalsmith (my wife), Foredom is the best. The
bits
> can be bought at either a local hardware store or from a supply
company
> like Rio Grande (I don't have an address handy, but can get one if
anyone
> is interested). There are other good brands as well. These are more
> expensive than Dremels, however (sometimes much more).
>
> The problem is that these are not portable tools--they either sit on a
> bench top, or hang inverted nearby and require AC line power. I
don't know
> of any cordless models off hand, but the art people in the local
metals
> program ought to know.
>
> Phil
>
> >I was pleasantly surprised to see this topic come up today.  I am not
> >using a dremel tool in the lab, but our wildlife health field crew
is for
> >doing necropsies on white-tailed deer.  They didn't want to spend the
> >money for a stryker saw.  These folks will probably want to use the
> >same instrument for cutting up seals, dolphins, whales, and sea
turtles
> >this summer.  Are there any negative implications for them
personally, or
> >specimen quality?  (I have managed to convince them to take chunks
that
> >aren't bigger than a megacassette, but they don't want to admit
that you
> >CAN collect a tissue that fits in a regular cassette IN THE FIELD.)
> >
> >Regards -
> >Carol
> >*****************************
> >Carol B.  McCollough, HT (ASCP)
> >Diagnostics & Histology Laboratory Manager
> >Maryland Department of Natural Resources
> >Fisheries Service
> >Cooperative Oxford Laboratory
> >904 S.  Morris Street
> >Oxford, MD 21654
>
> ****be famous! send in a tech tip or question***
> Philip Oshel
> Technical Editor, Microscopy Today
> PO Box 620068
> Middleton, WI  53562
> Voice: (608) 833-2885
> Fax: (608) 836-1969 (please make sure my name is on any fax)
> oshel@terracom.net
>
>
>
>
>
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