It might work on paraffin sections???  HOWEVER, I have made enough mistakes
by grabbing the wrong rinse bottle containing deionized water for rinsing
and BLEW APART MY FROZEN SECTIONS INTO PIECES OF UNREADABLE JUNK!  


At 07:26 PM 2/20/02 -0500, you wrote:
>Hi,
>    I have heard of a lab recently that does not use buffer for their IHC
>staining anymore. They have switched to deionized water. Now, of course I
>know not to do this but I was hard pressed to put into words why. I would be
>thrilled to hear a more eloquent explanation than what I am babbling on
>about.
>Amos Brooks
>
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>
>