No waste of time. The buffers are more important for the Ag-Ab Rx and marker
Rx's and stability than for other matters, unless the tissue is NOT fixed.
Then the buffer has more to do.

Fred

> ----------
> From: 	Amos Brooks
> Sent: 	Thursday, February 21, 2002 5:56 PM
> To: 	Monson, Frederick C.
> Cc: 	'List-HistoPath'
> Subject: 	Re: Spin on IHC buffers
> 
> Thanks,
>     Part of the senseless babbling I was referring to involves osmotic
> balance but it didn't make much sense the way I described it. I'm glad
> that
> I was on the same wavelength though.
>     The tissue is formalin fixed and paraffin embedded. So does that
> prevent
> the deleterious effects? If it does then people are wasting a lot of money
> on buffer. I for one am not quite ready to take such a chance but it bears
> consideration.
> Amos
> 
> ----- Original Message -----
> From: "Monson, Frederick C." 
> To: "'Amos Brooks'" 
> Cc: "'List-HistoPath'" 
> Sent: Thursday, February 21, 2002 4:26 PM
> Subject: RE: Spin on IHC buffers
> 
> 
> > Hi Amos,
> > One of my favorite sub-subjects.  Colligative properties of
> > solutions.  I think the words you want are "osmotic pressure".  If
> sections
> > are fresh-frozen and thick enough to house unsectioned cells, then just
> as
> > in a suspension of RBC's, diHOH will 'fill them to bursting', because of
> > "osmosis".   Same thing to the unfixed "ground substance" of connective
> > tissue.  Maybe I should issue forth on "Chromatin" and "Metachromasia".
> Ah
> > yes!  My alcohol of choice for dehydrating tissues stained with
> > metachromatic dyes is Tertiary ButAnal ("TBA" - Now why would anyone
> think
> > to  give the suffix of that word so much prominence?)  In any case, with
> > TBA, the metachromasia assuredly survives.  I've had some in Azure B
> (DNA
> > vs. RNA) mounted in Damar for 20 years and still see azure and purple.
> > Get those people free tickets to see "Osmosis Jones".
> > By the way, if the tissue is fixed, diHOH probably won't have any
> > deleterious effect on the results.  I mean, after we use the magic tap
> water
> > on the Schiff's Reagent, don't we rinse in diHOH before we enter the
> > alcohols.  No?  For Heaven's sake, why not!!!???!!!  Wouldn't we
> contaminate
> > the alcohol dilutions we made with diHOH?
> > Has anyone ever determined why the tap water in Duluth works better
> > on Schiff's than that in Minneapolis?  I never did hear how that debate
> was
> > resolved.  I missed most of it by becoming electron microscopic.
> > Well, Amos, I am taking tomorrow off, because I need to replace a
> > windshield wiper motor.  I'm starting at the exhaust pipe and I'm going
> to
> > work my way forward until I find that little bugger.  Then, after lunch,
> > while I revel in my mechanical skills, I'm going to watch the US team
> beat
> > the Russians in ice hockey and, hopefully, the Canadians beat the
> > Liliputians.
> >
> > My best wishes for all to have a peaceful weekend, especially our
> > neighbors who are sailing with the Navy, flying with the Air Force
> > (robotically with the Army) and sand flea hunting with the Marines.
> Smack!
> >
> > Fred Monson
> >
> > Frederick C. Monson, PhD
> > The best research
> > Center for Advanced Scientific Imaging
> > occurs before work
> > West Chester University
> > at the bench.
> > West Chester, Pennsylvania, USA, 19383
> > 610-738-0437
> > fmonson@wcupa.edu
> 
>