Brenda,
Just to clarify.  The skim milk is not blocking endogenous biotin, it is your
protein block for non-specific binding of the secondary.  The egg whites in the
skim milk method are doing the blocking of endogenous biotin.
Patsy

Brenda D Begg wrote:

> Thanks Teri,
> I found the skim milk method for blocking endogenous biotin in the archives.
> It helped alot.
>
> Brenda B.