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<DIV>Hi Richard</DIV>
<DIV>We are using Novocastra's mo-a-hu CD10, clone 56C6, Cat # NCL-CD10-270.
Working conc. is 0.6 ug Ig/ml. Ask your supplier to specify the Ig concentration
of the undiluted product - Novocastra has this information and
provides it on request (that's at least something - some companies provide the
Ig-conc of their product from the very beginning [that's where I like to shop],
others will never give you this information [that's where I start to have some
doubts about ... and prefer to go elsewhere]). Anyway, the antibody works
reasonably well on formalin-fixed tissue, although overfixation is absolutely
detrimental. We do a HIER using a microwave protocol and a 100 mM Tris buffer pH
9.5 (I know this is nonsense, but that's the way it 's published - and it works)
with 5% urea (your tissue will not like the urea, but the staining will be
better than after citrate buffer). Alternatively - and if you have money to burn
- you can probably try one of the commercially available high pH antigen
retrieval buffers.</DIV>
<DIV>Good luck!</DIV>
<DIV> </DIV>
<DIV>Andi Kappeler<BR>Institute of Pathology </DIV>
<DIV>University of Bern, Switzerland<BR></DIV></BODY></HTML>