When all else fails there are the CryoJane Tape-Transfer adhesive-coated
slides. Sections are bonded to the slide and will not, under vitually any
conditions, fall off the slide.
If you want the details contact Instrumedics and also visit our web site at
www.instrumedics.com

Bernice
schiller@instrumedics.com


----- Original Message -----
From: HistoNet Server <histonet@pathology.swmed.edu>
To: HistoNet Server <histonet@pathology.swmed.edu>
Sent: Friday, December 03, 1999 1:10 AM
Subject: Daily Digest


>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 03:45:33 -0600
> From: "Sabrina Dize" <Dize_Sabrina@piedmont.promina.org>
> Subject: Re: Case Distribution
>
> Hi all!
>
> Here at Piedmont we simply fill folders and distribute them evenly to
however
> many docs are reading that particular day. The only exception is when one
has
> done a lot of frozens on a case, then that case goes to the doc who read
the
> frozens. Still though he gets the same number of folders as the others.
Then
> later when the specials come out we try to distribute them as evenly as
> possible. ( we have a lot of pre-orderd specials).
>
> Lynn
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 05:45:56 -0600
> From: "O'Brien, Sue" <histo@bthosp.com>
> Subject: RE: Case Distribution
>
> Hi Amy, Sorry to hear about your dilemma. Currently, we also handle the
> split. The Pathology Director gave us certain guidelines (like, the
> pathologist that grossed the case usually reads it,  and they each have
> certain preferences for cases) which I go by when I look at the workload
at
> the end of the day. Then I assign cases to the doctor's according to these
> guidelines, keeping in mind as much an equal distribution of the work a
> possible. Do I blow it sometimes? You bet! Do they get all bent out of
shape
> about it? Not really (it doesn't happen that often). But for the most
part,
> they accept what is divvied up to them and I do not invest more than a few
> moments (tops) going though about 7-9 pages of work to be assigned. I
guess
> I am VERY fortunate to have a good working relationship with the
> Pathologist's I work for and experience mutual respect from all (a
CRITICAL
> formula, I think, for dividing up the cases to work). My next in charge is
> trained as a back-up for this (as we are never off at the same time). Hope
> this helps you,
> Sue O'Brien, Histology Supervisor
> Burdette Tomlin Memorial Hospital
> Cape May Court House, NJ  08210
> e-mail: histo@bthosp.com <mailto:histo@bthosp.com>
>
>
> -----Original Message-----
> From: Woodfin, Amy C [SMTP:AWoodfin@peacehealth.org]
> Sent: Wednesday, December 01, 1999 2:24 PM
> To: 'histonet@pathology.swmed.edu'
> Subject: Case Distribution
>
> Hi folks,
>
> Looking for some insight from hospital based surgical pathology labs
> on how
> your cases are distributed to your docs for microscopic evaluation.
> We are
> in our 5th evolution of case distribution methods and the docs are
> still not
> happy.  I would appreciate any information on: how many docs,
> average # of
> cases, who does the distribution, is equanimity of cases a goal in
> your
> distribution, and how you determine who gets what.  Any info will be
> GREATLY
> appreciated :-)
>
> Thanks!
> Amy Woodfin
> Pathology Supervisor
> St. Joseph Hospital
> Bellingham, WA  98225
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 05:46:20 -0600
> From: "Beverly Elswick"<beverly_elswick@bshsi.com>
> Subject: Information Systems for Pathology
>
>      Hello,
>      Our department is interested in evaluating information systems for
>      anatomic pathology. If anyone would like to share current experiences
>      or forward resources to contact, we would appreciate your help. If
any
>      vendors are "listening", please feel free to contact me via e:mail.
We
>      currently use Sunquest.
>      Thanks,
>      Bev Elswick
>      Bon Secours
>      Richmond, VA
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 06:31:17 -0600
> From: Cynthia A Delong <DELONG_CYNTHIA_A@LILLY.COM>
> Subject: Quotes on used or reconditioned processors
>
>
>
> Hello everyone in histoland,
>
> My boss has just asked me if I could get quotes on a processor.  Yeh!  No
> promises but I would like to have enough information and quotes to let her
see
> how much they are so that we can budget for next year.
>
> So, if anyone has any companies that can provide me with 1.  reasonable
prices
> and 2.  good technical service please let me know. Or processors that I
should
> stay away from.  I don't need any headaches.
>
> I would like it to be able to process at least 150 cassettes preferably
300
> and it needs to have vacuum on all stations.
>
> Thank you all for your help
> Cindy
> C.Delong@lilly.com
> 317-276-7635
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 07:00:59 -0600
> From: Limbic Lady <cxb41@po.cwru.edu>
> Subject: artery section with stainless stent
>
> A friend asked if anyone had suggestions on how to section an artery
> with a stainless steel stent in place.  I had not idea, as we had
> tried sectioning dacron blood vessels.
>
> Would anyone on this list have suggestions on how to approach this?
> Taking the stent out is not an option.
>
> THANKS!
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 07:13:19 -0600
> From: TABrecken@aol.com
> Subject: FSH to host NSH Region III in Orlando 2000
>
> Florida Society for Histotechnology will be hosting the NSH Region III
> meeting in Orlando, Florida March 16,17 & 18, 2000.  The program has been
> mailed.  If you need additional information, please send me an e-mail and
I
> can forward the program and registration form to you.
>
> Thanks,
>
> Tonia Breckenridge
> FSH President
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 07:24:33 -0600
> From: "Roberta Horner" <rjr6@psu.edu>
> Subject: NSH self-assessment booklets and disks
>
> There are two of us here that are planning on taking the HTL exam next
> spring.  I was wondering if anyone has used the NSH self-assessment
booklets
> and disks.  I have seen the booklets and am definitely planning on getting
> them but what are on the disks and are they worth it?
> Thanks in advance
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 07:24:59 -0600
> From: "O'Brien, Sue" <histo@bthosp.com>
> Subject:
>
> Thank-you to all who replied to my weekend coverage questionnaire.
Everyone
> from major  medical centers to rural community hospitals responded! I
> received 21 responses, and the answers were not what I expected, in some
> instances. For the most part, 300+ bed hospitals did provide coverage for
> Saturday only. This usually consisted of 8 hours or less (i.e. "until the
> work was done"). There were two (one 700 bed, one 400+) in this category
> that did NOT have any weekend service. Weekend services had been provided
> there in the past, but were cut along with the budget. Only one place had
> both Saturday and Sunday coverage. Generally, less than 300 bed hospitals
> either did not provide weekend service, or provided some sort of "call"
> coverage (e.g. only come in for STAT's, when needed). Out of all the
> respondents, only one place performed immunohistochemistries on the
weekend;
> only a few performed special stains (and then, only select stains like
> pneumocystis). Thank-you all again for taking the time to respond! If any
> would like a further breakdown, I have the responses compiled on an EXCEL
> spreadsheet (of course, leaving out originating hospital/service name),
> Sue O'Brien, Histology Supervisor
> Burdette Tomlin Memorial Hospital
> Cape May Court House, NJ  08210
> e-mail: histo@bthosp.com <mailto:histo@bthosp.com>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 08:01:01 -0600
> From: "Joan C. Yonchek" <yonchjc@ORTHO.UFL.EDU>
> Subject: Re:email address for Chemicon
>
>
> Chemicon International, Inc.
>
> custserv@chemicon.com
> techserv@chemicon.com
> www.chemicon.com
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 08:27:04 -0600
> From: Mary Lou Norman <mlm11@cornell.edu>
> Subject: rubber eye prosthesis
>
>
>
> I have always removed the rubber ball but I was wondering if you don't
have
> to. This horse must have been much loved to have received an ocular
> prosthesis. I'd like to know what others do with them.
>
> Also, does any one have a stash of beakers for the Shandon-Elliot Duplex
> Processor?  I would like some spare ones  and yes, it's under a hood.
>
> Thanks for the info,
>
> Mary Lou
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 08:40:19 -0600
> From: Dawn MacKinley <dmackinley@Upei.CA>
> Subject: Neuron Specific Enolase
>
> Hi there everyone;
> I need some advice from anyone who does immunoperoxidase
> for Neuron Specific Enolase on paraffin blocks on cat tissue. Can
> you tell me any idiosyncrasies it has. What dilution do you use, do
> you use Hier and what buffer do you use with that? Any
> suggestions will be greatly appreciated.
> Thanks
> Dawn MacKinley
> AVC
> PE
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 09:45:54 -0600
> From: Patsy.Ruegg@UCHSC.edu
> Subject: RE: NSH self-assessment booklets and disks
>
> Roberta,
> The disc are just computer forms of the books.  If you want an interactive
> computer program which allows you to answer the questions I suggest you
get
> the computer study guide put out by ASCP.  This was edited by Freida
Carson
> and I have had many people pass the exam using this.
> Patsy Ruegg
> ASCP Board of Registry  P.O. Box 12277  Chicago, IL  60612-0277  (312)
> 738-1336 E-mail  bor@ascp.org
>
> - -----Original Message-----
> From: Roberta Horner [mailto:rjr6@psu.edu]
> Sent: Thursday, December 02, 1999 6:17 AM
> To: Histonet
> Subject: NSH self-assessment booklets and disks
>
>
> There are two of us here that are planning on taking the HTL exam next
> spring.  I was wondering if anyone has used the NSH self-assessment
booklets
> and disks.  I have seen the booklets and am definitely planning on getting
> them but what are on the disks and are they worth it?
> Thanks in advance
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 09:58:26 -0600
> From: "Beverly Elswick"<beverly_elswick@bshsi.com>
> Subject: Interested in evaluating Laboratory Information Systems
>
>      Hello,
>      Our department is interested in evaluating information systems for
>      anatomic pathology. If anyone would like to share current experiences
>      or forward resources to contact, we would appreciate your help. If
any
>      vendors are "listening", please feel free to contact me via e:mail.
We
>      currently use Sunquest.
>      Thanks,
>      Bev Elswick
>      Bon Secours
>      Richmond, VA
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 10:10:57 -0600
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: RE: NSH self-assessment booklets and disks
>
> Very much so!!! I used everything NSH had. The discs provide similar
testing
> to the actual exam and are good practice. Sheehan and Carson were the only
> resources I used.
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
>
>
> -----Original Message-----
> From: Roberta Horner [SMTP:rjr6@psu.edu]
> Sent: Thursday, December 02, 1999 8:17 AM
> To: Histonet
> Subject: NSH self-assessment booklets and disks
>
> There are two of us here that are planning on taking the HTL exam
> next
> spring.  I was wondering if anyone has used the NSH self-assessment
> booklets
> and disks.  I have seen the booklets and am definitely planning on
> getting
> them but what are on the disks and are they worth it?
> Thanks in advance
> Roberta Horner HT
> Animal Diagnostic Lab
> Penn State University
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 10:31:44 -0600
> From: Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>
> Subject: Pay Scales
>
> Hello
> Does anyone know or have a reference for hourly wages for histologist.
> In addition,  does anyone now the going rate/scale for per diem
> histology services such as sectioning, staining, IHC etc....
> Much obliged
> Luis Chiriboga
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 11:01:04 -0600
> From: HACKERLAB@aol.com
> Subject: Re: Slding Microtomes
>
> Hacker Instruments Inc carries an entire line of sliding microtomes,
several
> of which are specially designed to section large specimens.
>
> Please forward your contact information (Name, address, fax#, etc.) so we
can
> provide you with detailed information.
>
> Please feel free to call with any questions.
>
> Sincerely,
>
> Dorothy Murphy
> National Sales Manager
> HACKER Instruments & Industries Inc
> 17 Sherwood Lane
> Fairfield, NJ 07004
> USA
> Tel: (973) 226-8450  or 1-800-4-HACKER
> Fax: (973) 808-8281
>  <A HREF="http://www.hackerinstruments.com/">Hacker Instruments &
Industries,
> Inc. - Home Page</A>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 11:20:42 -0600
> From: Corazon Bucana <bucana@audumla.mdacc.tmc.edu>
> Subject: fluorescent peroxidase substrate
>
> Just out of curiousity, is there a peroxidase substrate  for
> immunohistochemistry that forms an insoluble precipitate and at the same
> time is also fluorescent?  I am looking for something analogous to Fast
Red
> that is used for alkaline phosphatase.  The fluorescence provides more
> sensitivity.
>
> Thank you,
>
> Corazon D. Bucana
> *******************************************************
> Corazon D. Bucana, Ph.D.
> Department of Cancer Biology
> U.T. M.D. Anderson Cancer Center
> 1515 Holcombe Blvd.  Box 173
> Houston, Texas 77030
> Phone: (713) 792-8106
> FAX: (713) 792-8747
> Email:bucana@audumla.mdacc.tmc.edu
> FAX: (713) 792-8747
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 11:32:14 -0600
> From: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
> Subject: VEGF and bFGF
>
> Hi Histonetters,
>
> I need some help.  I'm trying to optimize Vascular Enothelial Growth
Factor
> (VEGF) and angiogenic factor bFGF, both from Oncogene.  I need these to
work
> on paraffin and a Ventana immunostainer.  If anyone has any experience out
> there with these I'd appreciate some info.  I'm not wedded to these
> particular antibodies, although the researcher may think we are!...so if
> there are other vendor's antibodies out there that people have used with
> sucess, I'd be interested in hearing about those too.
>
> Thanks for the help,
>
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
>
>
> (608)265-6596
> FAX: (608)263-1568
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 12:15:31 -0600
> From: "Vinnie Della Speranza" <dellav@musc.edu>
> Subject: MOHS Labs
>
> I would like to hear from anyone who is connected with a MOHS Laboratory
who
> could address adequate staffing for such a facility.
>
> I would like to learn how one would make this assessment. I am reluctant
to
> compare FTE's to cases, because Case #'s do not necessarily reflect the
amount
> of work performed. Do MOHS labs track the # of blocks frozen / cut? or
some
> other criteria reflective of workload ?
>
> If you are not associated with a MOHS lab but can point me to someone I
might
> talk to, I would be grateful.
>
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue
> Suite 309
> Charleston, SC  29425
> ph:  (843) 792-6353
> fax: (843) 792-8974
> email: Dellav@musc.edu
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 12:16:15 -0600
> From: AndreaH@imclone.com
> Subject: Ki-67
>
>
> Where do people buy their Ki-67?  I purchased a monoclonal that is
supposed
> to work on frozens as well as a polyclonal from DAKO and both were awful.
>
> Andrea T. Hooper
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 12:42:10 -0600
> From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
> Subject: RE: fluorescent peroxidase substrate
>
> Corazon,
> I think Vector has Texas Red which is similar to New Fuchsin.  I believe
it
> is insoluable in alcohol in xylene, is uded with alk-phos detection
systems
> and can be viewed under light and IF microscopes.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
>
>
>
>
>
>
>
>
> > -----Original Message-----
> > From: Corazon Bucana [SMTP:bucana@audumla.mdacc.tmc.edu]
> > Sent: Thursday, December 02, 1999 11:11 AM
> > To: histonet@pathology.swmed.edu
> > Subject: fluorescent peroxidase substrate
> >
> > Just out of curiousity, is there a peroxidase substrate  for
> > immunohistochemistry that forms an insoluble precipitate and at the same
> > time is also fluorescent?  I am looking for something analogous to Fast
> > Red
> > that is used for alkaline phosphatase.  The fluorescence provides more
> > sensitivity.
> >
> > Thank you,
> >
> > Corazon D. Bucana
> > *******************************************************
> > Corazon D. Bucana, Ph.D.
> > Department of Cancer Biology
> > U.T. M.D. Anderson Cancer Center
> > 1515 Holcombe Blvd.  Box 173
> > Houston, Texas 77030
> > Phone: (713) 792-8106
> > FAX: (713) 792-8747
> > Email:bucana@audumla.mdacc.tmc.edu
> > FAX: (713) 792-8747
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 12:42:43 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: fluorescent peroxidase substrate
>
> On Thu, 2 Dec 1999, Corazon Bucana wrote:
>
> > Just out of curiousity, is there a peroxidase substrate  for
> > immunohistochemistry that forms an insoluble precipitate and at the same
> > time is also fluorescent?
>
>   Yes. The procedure known as TAT (tyramide amplification
>   technique) or CARD (catalysed reporter deposition) is a
>   sensitive method for peroxidase. It generates a fluorescent
>   product that is covalently bound to the tissue at sites
>   of enzymatic activity. The chromogen is a compound made by
>   linking tyramine to a fluorochrome. In the presence of
>   peroxidase and its substrate, hydrogen peroxide, the
>   tyramine half of the chromogen molecule is oxidized to
>   a highly reactive free radical, which immediately reacts
>   with and binds to protein (probably mainly to tyrosine)
>   in the tissue. Simple methods for synthesizing fluorescently
>   labelled tyramides are described by Hopman,AHN et al (1998)
>   in J Histochem Cytochem 46:771-777.
>
>   I haven't tried this myself, but the published pictures
>   are impressive. It is used more for in situ hybridization
>   than for imunohistochemistry, but is suitable for any
>   technique in which peroxidase activity needs to be
>   localized.
>
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1
>    E-mail: kiernan@uwo.ca
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 13:20:33 -0600
> From: Nancy.Cardwell@mcmail.vanderbilt.edu
> Subject: Microtome Heidelberg
>
>
>
> Histonetters,
>
> We have acquired a free microtome which we really need.  Of course, it's
> missing
> the manual and we're having a very hard time figuring it out.  It is a
Microme
> Heidelberg - HM 330.  If anybody has any suggestions of a company that
> services
> it or a copy of a manual they would be willing to fax we would greatly
> appreciate it.
>
> I sent a similiar message yesterday and it has yet to appear on the Net so
if
> there is suddenly a duplicate, I apologize in advance.
>
> Thanks,
> Nancy Cardwell
> Plastic Surgery Research
> Vanderbilt University Medical Center
> Nashville, TN
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 13:21:21 -0600
> From: Phyllis Davie <pdavie@phenopath.com>
> Subject: Re: email address for Chemicon
>
> Chemicon International, Inc.
>  e-mail:  custserv@chemicon.com
>
> >Can anyone supply me with the email address of Chemicon International,
Inc.
> >Thanks in advance
> >Ann Samways
> >
> >Ann Samways,
> >Histology Unit,
> >Dept of Stomatology,
> >School of Dentistry,
> >P.O. Box 647,
> >Dunedin.
> >New Zealand
> >
> >Phone (O3) 4797079
> >Fax   (03) 4790673
> >Email-- ann.samways@stonebow.otago.ac.nz
> >
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 13:54:24 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: Microm microtome
>
> I believe that Microm has just merged with Richard Allan, give
> them a call to see if they can access your manual.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 13:54:57 -0600
> From: Dennis Sullivan <dennis.sullivan@home.com>
> Subject: Re: Ki-67
>
> InnoGenex has two different Ki-67 monoclonals.
>
> Ki-67 : 2D3 clone Mouse IgG1 -
> Purified cat # AM-2285-11
> Unpurified cat # AM-2285-01
>
> Ki-67 : 7C9 clone Mouse IgG1 -
> Purified cat # AM-2286-11
> Unpurified cat # AM-2286-01
>
> order- 1.877.IGX.INFO
>
> Hope this helps.
> Dennis Sullivan
> InnoGenex
>
> http://www.InnoGenex.com/
>
> AndreaH@imclone.com wrote:
> >
> > Where do people buy their Ki-67?  I purchased a monoclonal that is
supposed
> > to work on frozens as well as a polyclonal from DAKO and both were
awful.
> >
> > Andrea T. Hooper
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 14:23:23 -0600
> From: Simon Smith <SSimon@skeletech.com>
> Subject: RE: Hematoxylin
>
> Sorry to resurrect an old thread, but I new I had read the following
> somewhere and finally tracked it down to my prehistoric copy of Gurrs
> encyclopaedia of stains.
>
> HAEMATOXYLIN AND HAEMATEIN
>
> Haematoxylin is a natural colouring matter obtained from the wood of a
> species of tree, the Haematoxylon campechianum, Linn which is indigenous
to
> Mexico but is cultivated in the West Indies. It is not itself a dye, but
> owes its tinctorial properties to the formation of one of its oxidation
> products, haematein. Confusion has sometimes arisen between haematoxylin
and
> a crude resinous substance, known as hematoxylin, sometimes referred to as
> haematoxylin, which was at one time used in pharmacy and is still
> extensively used in the tanning industry. This crude product is obtained
by
> boiling logwood chips with water, filtering off the aqueous extract and
> evaporating it to dryness. Another crude product sometimes confused with
> haematein, is hematein, often referred to as haematein in the tanning
trade;
> this is produced by the oxidation of the aqueous extract of logwood chips.
> Haematoxylin as used in biology is obtained by extracting the crude
product,
> hematoxylin, with ether, while haematein, as already indicated is an
> oxidation product of haematoxylin. Although haematoxylin is used
extensively
> as a biological stain, and is, as Conn, H. J. (1953) points out, of great
> importance in that sphere and is as valuable to the cytologist and
> histologist as methylene blue is to the bacteriologist; compared with the
> crude product hematoxylin, used in industry, it is manufactured only on a
> small scale: hematoxylin is cheap and easy to prepare, costs a fraction of
> the price of haematoxylin, and is ordered by the ton, whereas a demand for
a
> kilo of the latter product by a biological laboratory, as readers will
know,
> is by no means considered small and would be sufficient for the
requirements
> of a reasonably large laboratory for some considerable time. It is
probable
> that the haematoxylins first produced in America during the First World
War
> mentioned by Conn, H. J. (1953) as having been found very crude and
> unsatisfactory, were in fact the crude aqueous extracts, known as
> hematoxylin, which were supplied through misunderstanding, to biologists,
as
> haematoxylin: the latter word is spelled "hematoxylin" in America, so that
> such an error could quite easily occur there.
>
> Can anyone throw any light on the process currently used to convert lumber
> into the stain we all know and love?
>
> Simon Smith B.Sc. AIBMS
> Associate Scientist
> Skeletech Inc
> 22002 26th Avenue SE Suite 104
> Bothell
> WA 98021
>
> Voice 425 424 BONE(2663)    Fax 425 424 2600
> Ssmith@skeletech.com <mailto:Ssmith@skeletech.com>       www.skeletech.com
>
>
> -----Original Message-----
> From: jim [mailto:jim@proscitech.com.au]
> Sent: Wednesday, November 17, 1999 5:54 AM
> To: 'Philip Oshel'; gfenn@bdhinc.com
> Cc: histoNet@pathology.swmed.edu
> Subject: RE: Hematoxylin
>
> North Americans use Hematoxylin and British/Australians use
> Haematoxylin. I
> expect that Heamatoxylin is a misprint. I lived in North
> America for some years
> and learned English as my second language.
> I like the often more phonetic American spellings better
> (and confuse the two
> versions), but how do we reconcile Hematoxylin, when the
> originating tree
> belongs to the genus Haematoxylon.
> I won't start a war over that, but think that Hematoxylin
> should be
> illegitimate until Botanist can be convinced to rename the
> genus to
> Hematoxylon.
> Cheers
> Jim Darley
> ProSciTech                 Microscopy PLUS
> PO Box 111, Thuringowa  QLD  4817  Australia
> Ph +61 7 4774 0370  Fax:+61 7 4789 2313
> service@proscitech.com
> Great microscopy catalogue, 500 Links, MSDS, User Notes
>                       www.proscitech.com
>
> On Wednesday, November 17, 1999 1:25 PM, Philip Oshel
> [SMTP:oshel@terracom.net]
> wrote:
> > >P.S. Anyone care to question the correct spelling of
> Haematoxylin or
> > >Heamatoxylin ?
> > >Gordon
> >
> > Haematoxylin. It's a now-separated diphthong like
> aeroplane or
> > aencyclopedia used be, or aeolian harp (not the lager).
> > (Diphthong, noun, from the ancient Greek "Help! my
> tongue's stuck in the
> > back of my teeth!)
> >
> > Phil
> >
> > ****be famous! send in a tech tip or question***
> > Philip Oshel
> > Technical Editor, Microscopy Today
> > PO Box 620068
> > Middleton, WI  53562
> > USA
> > Address for FedEx, UPS, etc.:
> > Dept. of Animal Health and Biomedical Science
> > University of Wisconsin
> > 1656 Linden Drive
> > Madison, WI  53706 - 1581
> > Voice: (608) 463-4162 days, (608) 833-2885 evenings
> > Fax: (608) 836-1969 (please make sure my name is on any
> fax)
> > oshel@terracom.net
> > or
> > peoshel@facstaff.wisc.edu
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 14:43:19 -0600
> From: Patsy.Ruegg@UCHSC.edu
> Subject: RE: VEGF and bFGF
>
> Linda,
> I use vegf and fgfb both from santa cruz on paraffin processed tissues. i
> fix with zinc formal but i amagine 10% NBF would work, but may require
more
> stringent antigen retriveal.  all i do is a short proteinase k digestion
(5
> min.) before staining.  i use a 1:200 dilution and a strept-avidin biotin
> hrp detection system (DAKO)
> Patsy
>
> - -----Original Message-----
> From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu]
> Sent: Thursday, December 02, 1999 10:18 AM
> To: 'Histonet'
> Subject: VEGF and bFGF
>
>
> Hi Histonetters,
>
> I need some help.  I'm trying to optimize Vascular Enothelial Growth
Factor
> (VEGF) and angiogenic factor bFGF, both from Oncogene.  I need these to
work
> on paraffin and a Ventana immunostainer.  If anyone has any experience out
> there with these I'd appreciate some info.  I'm not wedded to these
> particular antibodies, although the researcher may think we are!...so if
> there are other vendor's antibodies out there that people have used with
> sucess, I'd be interested in hearing about those too.
>
> Thanks for the help,
>
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
>
>
> (608)265-6596
> FAX: (608)263-1568
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 14:44:03 -0600
> From: Joyce Friedland <joycefr@frontiernet.net>
> Subject: Tissue Adhesion for Immunos
>
> What methods are all the experts out there using to keep tissue from
> loosening, folding over and falling off during Immunoperoxidase
> procedures?? The biggest problem is during antigen retrieval using citrate
> buffer in the microwave.
>
> Thanks,
> Joyce
>
> ************************************
> *  Joyce Friedland                 *
> *  joycefr@frontiernet.net         *
> *  www.frontiernet.net/~joycefr    *
> ************************************
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:12:59 -0600
> From: "P. Emry" <emry@u.washington.edu>
> Subject: unstained slide storage
>
> Hi,
>
> I have made extra slides (paraffin embed) which I may want to use in the
> future.  Is it possible to cover them with crystalmount or some other
> product to protect them in storage?
>
> Can they be used for ihc after the mounting media is removed?  I would
> like to move the extra slides from the black boxes into
> storage, one slide next to the other.  Will they be damaged if there is no
> coverage?  Wax paper?  I'm guessing.
>
> Thanks for your time.
>
> Trisha
> U of WA, Seattle
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:13:24 -0600
> From: joyce judge <joycejudge@yahoo.com>
> Subject: archives
>
> Could someone please tell me how to access the
> archives.
> joyce judge
>
> __________________________________________________
> Do You Yahoo!?
> Thousands of Stores.  Millions of Products.  All in one place.
> Yahoo! Shopping: http://shopping.yahoo.com
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:13:55 -0600
> From: "Bonnie Whitaker" <bwhita@casper.med.uth.tmc.edu>
> Subject: mouse skeleton for Alizarin red
>
> HI,
> Does anybody have a good protocol for staining mouse skeletons in situ
with
> alizarin red (also removal of tissue)?
> I have a researcher here who needs this.
> Thanks,
> Bonnie Whitaker
> UT--Houston
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:52:54 -0600
> From: "Ronnie Houston" <wee_rory@hotmail.com>
> Subject: Bond-a-glass
>
> Need help from colleagues in the UK.
>
> A co-worker has mentioned a resin "BOND-A-GLASS" (much less toxic than
MMA)
> that has been used in the UK for embedding undecalcified bone. Can anyone
> supply more info, any references if the work has been published, the name
> address and phone # of the manufacturer, and any distributor in the US?
>
> Thanks
> Ronnie Houston
> Cytochemistry & Molecular Pathology
> Texas Scottish Rite Hospital for Children
> Dallas
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:53:38 -0600
> From: "Bonnie Whitaker" <bwhita@casper.med.uth.tmc.edu>
> Subject: mouse skeletons stained with Aliz. red
>
> Hi,
> Does anybody have a good protocol for staining whole mouse skeletons with
> alizarin red (also removal of tissue)?
> I have a researcher here who needs this.
> Thanks,
> Bonnie Whitaker
> UT--Houston
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:54:25 -0600
> From: "Tarpley, John" <jtarpley@amgen.com>
> Subject: RE: mouse skeleton for Alizarin red
>
> Bonnie,
> There are many variations of the basic protocols around. The two I'd
> recommend, of course :),  are Miller, DM and Tarpley, JE. Biotechnic &
> Histochemistry 71: 79-83, 1996  and Tarpley, JE. Biotechnic and
> Histochemistry 74: 116-118, 1998.
>
> John Tarpley 15-2-B
> Associate Scientist
> Specialist Image Analysis & Immunohistochemistry
> Amgen Inc
> One Amgen Center Drive
> Thousand Oaks, CA  91320
>
>
> > ----------
> > From: Bonnie Whitaker[SMTP:bwhita@casper.med.uth.tmc.edu]
> > Sent: Thursday, December 02, 1999 1:03 PM
> > To: histonet
> > Subject: mouse skeleton for Alizarin red
> >
> > HI,
> > Does anybody have a good protocol for staining mouse skeletons in situ
> > with
> > alizarin red (also removal of tissue)?
> > I have a researcher here who needs this.
> > Thanks,
> > Bonnie Whitaker
> > UT--Houston
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 15:54:56 -0600
> From: "Shotsberger-Gray, Wanda" <WandaShotsberger-Gray@hmhs.com>
> Subject: Re: Bone histomorphometric image analysis systems
>
> Hello All,
> I am taking a course in image analysis, and have "been exposed" through
this
> course, to three different image programs.  The one I found easiest to use
> is called MetaMorph.  One of the guys in the class is a rep for the
company,
> which was a big help because he was able to show us the ins and outs of
all
> the programs.  (Believe it or not, he was not biased).  Anyway, of the
> three, MetaMorph, ImagePro and Optimus, MetaMorph comes out on top for
ease
> of use and features included, and ImagePro has the most features, but can
be
> difficult to figure out.  If anyone is interested, the guy in my class is
> Mike Davis, and the number I have on his card is 800-976-7562.
> The class has been a ball, by the way.
> Wanda Shotsberger
> Harris Methodist Hospital
> Fort Worth TX
>  ----------
> From: Mary Stevens
> To: jlinda@ces.clemson.edu; histonet@pathology.swmed.edu
> Subject: Re: Bone histomorphometric image analysis systems
> Date: Wednesday, November 17, 1999 3:47PM
>
> I've also tried Image pro, but only on a Mac - if you use a Mac, don't
> invest in it - there were several bugs I found which the company stated
they
> had no intention of fixing for Mac's.  PC version is OK - but again, not
> specific for bone - compared to the Osteomeasure system.
>
> Mary
>
>
> >>> Linda Jenkins <jlinda@ces.clemson.edu> - 11/17/1999 11:11 AM >>>
> Hi, Gayle,
> I was surprised no one mentioned the Image Pro software.  Great
> and powerful stuff! Of course, I only use about 10 of the 200 or so
> features it contains.  I think most microscope companies carry this  and
> will readily quote you prices.
> John Tarpley might also have a few favorites in this area.
> Happy shopping!
> Linda
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> Clemson, SC
> **********************************
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 16:47:44 -0600
> From: "MacDonald, Jennifer" <jmacdonald@sach.org>
> Subject: RE: Case Distribution
>
> The histotechs match the requistions with the slides and leave them on a
> table for the pathologists to pick up.   They can fight amongst themselves
> if the distribution isn't "fair".  This has worked very well for us.
>
>
>
> > Looking for some insight from hospital based surgical pathology labs on
> > how
> > your cases are distributed to your docs for microscopic evaluation.  We
> > are
> > in our 5th evolution of case distribution methods and the docs are still
> > not
> > happy.  I would appreciate any information on: how many docs, average #
of
> > cases, who does the distribution, is equanimity of cases a goal in your
> > distribution, and how you determine who gets what.  Any info will be
> > GREATLY
> > appreciated :-)
> >
> > Thanks!
> > Amy Woodfin
> > Pathology Supervisor
> > St. Joseph Hospital
> > Bellingham, WA  98225
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 16:48:13 -0600
> From: "R.Wadley" <s9803537@pop3.unsw.edu.au>
> Subject: Re: artery section with stainless stent
>
> Hi,
>
> Your best, & probably only bet is to embed in resin (araldite) & take it
> off to you local geology lab.  There a section can be cut, mounted on a
> slide & ground down to a suitable thickness.  Then you can stain the
> section.  This process wastes a lot of tissue, but you should be able to
> get 2 - 5 sections per block.
>
> If you are lucky & the stent is extremely fine you may be able (after
> embedding in resin) to section with a hardened steel blade.  The results
> will not be as good as using the grinding & polishing technique, & may
> distort the position of the stent, but you can with care get more sections
> to look at.
>
> I seem to remember a thread on this sometime ago.
>
> Regards
>
> Rob W.
>
> At 07:53 12/02/1999 +0100, you wrote:
> >A friend asked if anyone had suggestions on how to section an artery
> >with a stainless steel stent in place.  I had not idea, as we had
> >tried sectioning dacron blood vessels.
> >Would anyone on this list have suggestions on how to approach this?
> >Taking the stent out is not an option.
> >THANKS!
>
>
> R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
> Laboratory Manager
> Cellular Analysis Facility
> School of Microbiology & Immunology
> UNSW, New South Wales, Australia, 2052
> Ph (BH) +61 (2) 9385 3517
> Ph (AH) +61 (2) 9555 1239
> Fax +61 (2) 9385 1591
> E-mail r.wadley@unsw.edu.au
> www http://www.micro.unsw.edu.au/caf.html
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 16:48:47 -0600
> From: "MacDonald, Jennifer" <jmacdonald@sach.org>
> Subject: RE: Case Distribution
>
> WOW!!!! Sure glad I have the pathologists that I have!
>
> > ----------
> > From: Tapper, Sheila[SMTP:STapper@smdc.org]
> > Sent: Wednesday, December 01, 1999 9:05 PM
> > To: 'Woodfin, Amy C'; 'histonet@pathology.swmed.edu'
> > Subject: RE: Case Distribution
> >
> > Amy,
> > You have asked a question I have long been too embarrassed to ask!!  My
> > pathologists insist that we administer a complicated, TIME CONSUMING
split
> > process for them.  When I informed them that the process they have
> > determined to equally distribute slides would delay the delivery of
their
> > slides by 30-45 minutes every day (No kidding!), they were fine with
> > that!!!
> > The answer I received when I protested was "It makes better financial
> > sense
> > for a histotech to spend the time to divide the work, and distribute
than
> > a
> > pathologist, who has many other responsibilities to take the time to
> > figure
> > it out.  I have a 5-page procedure.  I have so many variables to use in
> > computing the split, that I had to create a worksheet to be able to
> > administer it!!!!  When I took the information from the worksheet, and
put
> > it into an Excel spreadsheet.  I charted averages, daily workloads, and
> > distribution.  I presented it to the Medical Director; he was not
> > impressed...the current system was "working" well for them.  This,
despite
> > the fact it has created a HUGE rift between the techs and the
> > pathologists.
> > It is difficult to maintain a respectful working relationship when the
> > respect is only expected to go one way.  The funny part is...if we
should
> > every inadvertently make a mistake in dividing their work, they know
> > immediately!!!!  They will come STOMPING and in one case SCREAMING into
my
> > lab to belittle us for making a mistake.  My only question to them...How
> > would you know we had made a mistake, if you weren't mentally splitting
> > the
> > workload yourselves.  Needless to say...we are at an impasse, and still
> > performing the split.  My new lab director is supporting me, but this
> > issue
> > is obviously a symptom of a much larger issue.  I work with a group of
> > pathologist that cannot communicate amongst one another.  They are
certain
> > that it is reasonable to have us assign their work to them.  I have
> > threatened to bring in a phlebotomist to divide our blocks up so that no
> > one
> > histotech cuts more blocks than the other!!!!  They didn't see the humor
> > there either.
> >
> > Sorry for the rant.  I just brought the topic up to the Medical Director
> > last week - I lost!  He has devised a new scheme though...he wants us to
> > distribute slides in "discreet linear packages" (consecutive cases -
this
> > is
> > his terminology.  I guess all the time he saves while we do the split
> > allows
> > him to create new catch phrases for slides on a tray!)  I'm sorry.  I
> > better
> > stop.  I am embarrassing my organization.  If however, anyone would like
a
> > copy of my Distribution of Slides procedure, I would be happy to share
it
> > with you.  It could be a real laugh for those of you who are blessed
with
> > sane, reasonable pathologists who don't seem to thrive on power, and
> > appreciate how hard the techs work.  I will apologize in advance to any
> > pathologist on the net that I may have offended.  I have to say I am not
> > impressed with the group I am working for now.
> >
> > Sheila Tapper
> > Anatomic Pathology Team Leader
> > SMDC Health System
> > Duluth, MN
> > Stapper@smdc.org <mailto:Stapper@smdc.org>
> >
> >
> > -----Original Message-----
> > From: Woodfin, Amy C [SMTP:AWoodfin@peacehealth.org]
> > Sent: Wednesday, December 01, 1999 1:24 PM
> > To: 'histonet@pathology.swmed.edu'
> > Subject: Case Distribution
> >
> > Hi folks,
> >
> > Looking for some insight from hospital based surgical pathology labs
> > on how
> > your cases are distributed to your docs for microscopic evaluation.
> > We are
> > in our 5th evolution of case distribution methods and the docs are
> > still not
> > happy.  I would appreciate any information on: how many docs,
> > average # of
> > cases, who does the distribution, is equanimity of cases a goal in
> > your
> > distribution, and how you determine who gets what.  Any info will be
> > GREATLY
> > appreciated :-)
> >
> > Thanks!
> > Amy Woodfin
> > Pathology Supervisor
> > St. Joseph Hospital
> > Bellingham, WA  98225
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 16:49:17 -0600
> From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
> Subject: RE: Tissue Adhesion for Immunos
>
> Joyce,
> when we used the microwave oven to treat our slides, we placed the tissue
on
> positive charged slides.  The waterbath was filled with distilled water
only
> (no gelatin or other adhesive) and kept the slides in a 60 degree oven
> overnight.  We still had some problems with bone marrows and some fatty
> tissue such as breast.  we changed to steaming our slides.  We get good
> results and it is less damaging to the tissue.  Hope this helps.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
> > -----Original Message-----
> > From: Joyce Friedland [SMTP:joycefr@frontiernet.net]
> > Sent: Thursday, December 02, 1999 2:43 PM
> > To: histonet@pathology.swmed.edu
> > Subject: Tissue Adhesion for Immunos
> >
> > What methods are all the experts out there using to keep tissue from
> > loosening, folding over and falling off during Immunoperoxidase
> > procedures?? The biggest problem is during antigen retrieval using
citrate
> > buffer in the microwave.
> >
> > Thanks,
> > Joyce
> >
> > ************************************
> > *  Joyce Friedland                 *
> > *  joycefr@frontiernet.net         *
> > *  www.frontiernet.net/~joycefr    *
> > ************************************
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 17:41:28 -0600
> From: Lynn Gardner <lynn-gardner@uiowa.edu>
> Subject: New job
>
> Dear histonetters;
>
> Just wanted to say thank you to all of you who e-mailed and phoned me with
> information about jobs in histology. I was actually able to find a job
> right here at the University only I am now a total researcher. I will be
> working with breast, prostate, ovarian, placenta and other tissues both
> normal and cancerous. I will be working with cells and tissues so I am
very
> excited.
>
> For those of you who would like my new address and phone number here it
is:
>
> Lynn Gardner
> University of Iowa Health Care
> Department of Anatomy and Cell Biology
> 51 Newton Road
> 1-100 BSB
> Iowa City, IA 52242
>
> Phone: 319-335-7560
> Fax: 319-335-7770
>
> Thanks again everyone!
> Lynn Gardner
>
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 18:00:01 -0600
> From: DDittus787@aol.com
> Subject: Re: Ki-67
>
> I get my Ki-67 from Zymed, run at 1:100 with retreival in citrate buffer,
> microwaved,
> on the Ventana stainer for 32 min,with wonderful results. Only drwaback to
> any Ki-67
> clones I have used, (and i have tried lots) slides are affected by
oxidation,
> and need to be relatively fresh.              Dana Dittus
>                                          Abington Memorial Hospital
>                                          Histology Supervisor
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 18:00:47 -0600
> From: DDittus787@aol.com
> Subject: Re: Tissue Adhesion for Immunos
>
> How long are you retrieving for and are you using pos charge slides?
> Sometimes over-retrieval is the problem, or the slides are not baked for
long
> enough time. It is a recurrent problem and I can't wait to hear all the
> possible solutions on this one.
>                                                      Dana
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 19:06:52 -0600
> From: denise M m Long-Woodward <denisew2@juno.com>
> Subject: CD31?
>
> Hi knowledgable Histonet surfers!   One of my PostDocs wants me to ask
> what other researchers are doing to label endothelial cells in mouse
> tissues (frozen and/or paraffin). Please be specific about company,
> clone, dilution, digestion/HIER techniques used.......etc.  We have been
> using Pharmigen  Rat anti-mouse CD31 (clone MEC 13.3) and
> mouse anti-human CD31 (WM59) with success but with additional lymphocytic
> populations also labelling.   We have tried Ulex europeus (Vector,
> Biotinylated) but it doesn't seem to label mouse vessels, only human.
> Any suggestions would be appreciated.
> denise woodward
> boston, ma
>
>
> ___________________________________________________________________
> Why pay more to get Web access?
> Try Juno for FREE -- then it's just $9.95/month if you act NOW!
> Get your free software today: http://dl.www.juno.com/dynoget/tagj.
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 20:12:57 -0600
> From: theresa lisiewski <theresalisiewski@yahoo.com>
> Subject: Grossing of specimens
>
>
> Hi!Could someone please post the CLIA regs concerning
> histotechs grossing specimens. Also what constitutes a
> high complexity histology lab? Does routine special
> staining fall into that category ? Would a person with
> a high school diploma and trained on the job in histo
> more than fifteen years ago be eligible to continue to
> work in the department doing special stains.(Not
> immunos).   Thank you..   Theresa
> __________________________________________________
> Do You Yahoo!?
> Thousands of Stores.  Millions of Products.  All in one place.
> Yahoo! Shopping: http://shopping.yahoo.com
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 22:16:03 -0600
> From: Katri Tuomala <katri@istar.ca>
> Subject: Re: archives
>
> Hi Joyce,
>
> Archives are down due to a Florida hurricane damage. Hopefully it will
> be back in the new year! Katri.
>
>
> ----------------------------------------------------------------------
>
> Date: 2 Dec 1999 22:16:36 -0600
> From: Katri Tuomala <katri@istar.ca>
> Subject: Re: Tissue Adhesion for Immunos
>
> Hi Joyce,
>
> I use microwave retrieval for 15 minutes and used to have a lot of
> problems with losing sections. Since I started using Plus slides and
> distilled water in my waterbath (nothing added to it) I hardly ever have
> to repeat cases due to loss of sections. I dry all my slides overnight
> at 45C oven.
> Hope this helps, I know how frustrating it is...
> Katri
>
> Katri Tuomala
> Anatomic Pathology
> St.Joseph's Hospital
> Hamilton, Ontario, Canada
>
>
> Here are the messages received yesterday!
>
>