Hi Denise,
                  I'm presently working on a mouse model of angiogenisis
and I have been asked to try a variety of endothelial markers. I have been
using vWF (von Willibrands factor), CD31 and v-cam-1 and I-cam-1. vWF
stains endothelial cells very well in formalin fixed paraffin embedded
mouse skin tissue. CD31 works on paraffin tissues but what I think the
researcher need is a marker for neovascularization, something like a mouse
VEGF which I don't know of any? So far I have not been able to shead light
on the researchers studies with these markers, but I keep trying. As far as
the clone and things go I have that in a word table which I can't attach to
the histnet but if you like I can send it to your e-mail address. The I-cam
and V-cam and CD31 are from pharmingen and the ICAM and VCAM only seem to
work in frozen tissues. That's it in a nutshell let me know if you want the
rest of the info, hope it helps.

Jamie Erickson
Associate Scientist
Genetics Institute
Andover, MA 01810


>>> denise M m Long-Woodward <denisew2@juno.com> 12/02 3:11 PM >>>
Hi knowledgable Histonet surfers!   One of my PostDocs wants me to ask
what other researchers are doing to label endothelial cells in mouse
tissues (frozen and/or paraffin). Please be specific about company,
clone, dilution, digestion/HIER techniques used.......etc.  We have been
using Pharmigen  Rat anti-mouse CD31 (clone MEC 13.3) and
mouse anti-human CD31 (WM59) with success but with additional lymphocytic
populations also labelling.   We have tried Ulex europeus (Vector,
Biotinylated) but it doesn't seem to label mouse vessels, only human.
Any suggestions would be appreciated.
denise woodward
boston, ma


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