Hello Jamie,
A friend of mine stained xenographs with an anti-VEGF that recognizes both
mouse and human VEGF. She purchased this antibody from Oncogene.
Sorry it took so long, but my friend returned to Germany and it was
difficult to get the information.
Best Regards,
Matthew Ogdie
InnoGenex
(925)543-1414


-----Original Message-----
From: Jamie Erickson [mailto:JErickson@genetics.com]
Sent: Friday, December 03, 1999 8:10 AM
To: denisew2@juno.com; histonet@pathology.swmed.edu
Subject: Re: CD31?


Hi Denise,
                  I'm presently working on a mouse model of angiogenisis and
I have been asked to try a variety of endothelial markers. I have been using
vWF (von Willibrands factor), CD31 and v-cam-1 and I-cam-1. vWF stains
endothelial cells very well in formalin fixed paraffin embedded mouse skin
tissue. CD31 works on paraffin tissues but what I think the researcher need
is a marker for neovascularization, something like a mouse VEGF which I
don't know of any? So far I have not been able to shead light on the
researchers studies with these markers, but I keep trying. As far as the
clone and things go I have that in a word table which I can't attach to the
histnet but if you like I can send it to your e-mail address. The I-cam and
V-cam and CD31 are from pharmingen and the ICAM and VCAM only seem to work
in frozen tissues. That's it in a nutshell let me know if you want the rest
of the info, hope it helps.

Jamie Erickson
Associate Scientist
Genetics Institute
Andover, MA 01810


>>> denise M m Long-Woodward <denisew2@juno.com> 12/02 3:11 PM >>>
Hi knowledgable Histonet surfers!   One of my PostDocs wants me to ask
what other researchers are doing to label endothelial cells in mouse
tissues (frozen and/or paraffin). Please be specific about company,
clone, dilution, digestion/HIER techniques used.......etc.  We have been
using Pharmigen  Rat anti-mouse CD31 (clone MEC 13.3) and
mouse anti-human CD31 (WM59) with success but with additional lymphocytic
populations also labelling.   We have tried Ulex europeus (Vector,
Biotinylated) but it doesn't seem to label mouse vessels, only human.
Any suggestions would be appreciated.
denise woodward
boston, ma


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