Re: [IHC on frozen sections of brain]
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| From: | Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Barry, Lilith wrote:
>
> I use Vector ABC kit routinely on perfused brains and it works very well.
> The few times I have tried to use it on fresh-frozen sections, the
> background was very high. Does anybody know why?
> Lilith
>
I am also very pleased with the Vector kit(s). In perfused tissue
plasma (and other) proteins are washed out of the tissues while in fresh
frozen they remain, thus the higher background (I think).
Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane Piscataway, NJ 08854
voice: (732)-235-4583; fax -4029 e-mail: mcauliff@umdnj.edu
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