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---HistoNet Server <HistoNet@Pathology.swmed.edu> wrote:
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 02:15:53 -0600
> From: ALLISON@cardiff.ac.uk
> Subject: New postal regulations
>
> Mainly to UK histologists.
>
> Have you seen the new Postal Regulations for infectious substances
> which come into effect Jan 1st 1999?  Regs known as U.N. 602
> Obliquely related I know, but would be interested to know what
> packaging being used for fixed tissue coming to the lab by post. I am
> thinking particularly of commercial packaging and about the Post
> Office "return container" service.
>
>  My guess is that there would be some argument about whether tissue
> - - formalised or not, forgive the word - was infectious, especially
> given those little p's.
> Russ Allison, Wales
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 08:00:56 -0600
> From: "Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU>
> Subject: Autofluorescence
>
> Hello:
>
> I have just started doing immunostaining of muscle dissections.  The
tissue
> has been immersion fixed in 4% paraformaldehyde 0.2% Picric acid for
about 24
> hrs at 4C.  My primary antibodies are all fairly dilute at 1:3000 or
1:4000,
> secondary is at 1:400 and I am using CY3 at 1:600.  I have tried
ammonium
> chloride and sodium borohydride but still have considerable
background from
> the muscle fibers.  I was thinking of switching to a different
fixation.  Any
> suggestions would be appreciated.  Hope I have given sufficient info.
>
> Thanks in advance
>
> Michael
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 08:15:57 -0600
> From: "N.Aljehani" <MDQ96NA@sheffield.ac.uk>
> Subject: unscribe
>
> unscribe
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 09:01:01 -0600
> From: "Alan Bright" <Bright@dial.pipex.com>
> Subject: Re: sectioning thick frozens
>
> I cannot understand why so many Histonetters are having problems and
> avoiding thick frozen sections ?
>
> The main points to follow to achieve thick frozen sections  are:
>
> 1) Correct tissue temperature is most important, to cold will cause
> excessive cracking.
>
> 2) Brain must be sectioned between -8 to -12deg.C. & the knife
temperature
> should be around -10deg.C. colder than the tissue. By using this
method
> sections are cut up to 300 microns routinely.
>
> Merry Christmas to All,
>
>
> Alan Bright
>
> Bright Instrument Co.Ltd.
> St Margarets Way
> Huntingdon
> PE18 6EB
> England
>
> Tel No; 01480 454528
> Fax No;01480 456031
> Email ; Bright@dial.pipex.com
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 09:10:46 -0600
> From: "Suder, Joanne" <JC_Suder@fccc.edu>
> Subject: Happy Holidays!!
>
> To All Histonetter's
>
> May the joy of the holidays be with you. May the New Year bring
health and
> happiness to all
>
> Joanne Suder
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 10:00:43 -0600
> From: "Carpenter, Judith A." <Jude.Carpenter@vtmednet.org>
> Subject: Ventana/Biotek 500
>
> We have a virtually new Ventana/Biotek Techmate 500 for
> possible sale.  Is anyone interested and at what price ?
> Call/email me with offer.
> Jude
> Jude Carpenter,BS, HTL(ASCP)
> Chief Technologist/Surgical Pathology/Histology/Autopsy
> FAHC/MCHV Campus
> 111 Colchester Ave.
> Burlington,   VT  05401
> (802)656-5116
> jude.carpenter@vtmednet.org
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 11:15:42 -0600
> From: itsb9@juno.com (Scott C Taft)
> Subject: Processors
>
> We are also looking for a new processor. How many are out there on the
> market and who sells them?
>
> Thanks again.
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at
http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 11:16:05 -0600
> From: itsb9@juno.com (Scott C Taft)
> Subject: Microtomes
>
> We want to purchase a new microtome in our lab. How many different
> companies make microtomes now and who are they?
>
> Thank you
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at
http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 11:16:27 -0600
> From: "Harclerode, Donna" <DHarclerode@ligand.com>
> Subject: IHC on 50 micron brain sections
>
> Hi Lillith any anyone else who is interested,
> I used to work in a Neuroscience lab and we did 20-50 micron
sections on
> bird (and other animal) brain and flat sections of retina on a routine
> basis with wonderful results.  The free floating sections were stained
> in mircocentifuge tubes and rinsed in nets (there are many other ways
> out there) then mounted on slides. Each tube can be stuffed full of
> sections and  incubated in the fridge overnight in primary with
constant
> gentle agitation. Glass hooks made from Pasteur pipettes sealed and
bent
> with a Bunsen burner are ideal to handle sections. Paint brushes can
> also be used to transfer sections.
>
> I will be happy to fax anyone complete protocols.
>
> Donna Harclerode HT, HTL, (ASCP) QIHC
> Ligand Pharmaceuticals
> San Diego, CA
>
> PS. I provided PharMingen with the IHC protocols they printed in their
> 97 catalog if you can get a copy of that.
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Dec 1998 11:46:36 -0600
> From: "Mickie L. Johnson" <johnsom@shmc.org>
> Subject: Disposal of DAB
>
> We have just set up a Dako Immunostainer and are now trying on the
best and
> cheapest way to dispose of the DAB waste.  We can ship out the
liquid waste
> for $7-800.00 per 55 gallon drum or neutralize the waste or now use
a filter
> system from Nyad which costs about $300.00 per filter which will
handle up
> to 40 gallons.
>
> Does anyone out there know which is best. The filter requires a
peristaltic
> pump and stand which Nyad sells for $1700.00 which seems a bit
steep.  Can
> anyone help?
>
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