Hi there,

one of the user I am working with is having tough time to found a good
antibody for c-fos to use in cryosection of mouse brain (30micron size) in
immunofluorescence.

Any suggestion? should she use a different protocol?The protocol right now
is perfusion with PFA, sucrose, OCT,cutting, permebilization with
0.3%triton, blocking with serum and secondary A488.

thanks in advance

Valeria




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