Once the tissue is well fixed (24 hours in fixative?), storage in PBS or
in Fixative is not critical.  Storage in sucrose until it sinks will
ensure that freezing rate is not as critical, since smaller and fewer
crystals will form in the disruptive sucrose environment.  
 

Cordially,

Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten@myneurolab.com   
http://www.myneurolab.com 

 

 

________________________________

From: Frouwke Kuijpers [mailto:F.Kuijpers@science.ru.nl] 
Sent: Thursday, December 16, 2004 8:00 AM
To: Charles Scouten
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] storage of cryosections


Charles, a question:
 
When the tissue is emersed in sucrose 30% is the storage than less
critical because there are hardly any cristals formed?
 
Frouwke
 
F.J.Kuijpers-Kwant
Dept. Cellular Animal Physiology
University of Nijmegen
Toernooiveld 1 
6525 ED Nijmegen
frouwke@sci.kun.nl 
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