From: | "Anacko, Nancy L." |
Hi, A research tech is having a problem.....she is doing stains on fresh frozen brain that she recieves from a brain bank. The problem is ice-artifact on her sections/stains. She told me that she recieves the brain at -70 and then cuts them on a cryostat at -20. Any suggestions....I only work with fixed brain in parrafin. Thanks Nancy