Hi Carlos,
I don't quite understand what you mean by this pattern, but what I
frequently come accross with are some squamous epithelial cells from
technologist's fingers stuck on the slide while numbering and handling it.
They appear as blotches on or under your test sample and stain very strongly
with wide spectrum cytokeratin. Just a thought...

Katri

----- Original Message -----
From: "Carlos Defeo" 
To: 
Sent: Monday, December 09, 2002 12:06 AM
Subject: patchy pattern


Hi all:
I would like if you could give me any good explanation to the patchy pattern
that gives me distinct marks of broad spectrum citokeratin and also
cocktails of it on some cases on paraffin sections.Sections are  mount on
silanyzed slides.
I can#180#t give some usefull data like condition of fixation because I receive
the material as a paraffin block.In all cases I practiced antigen retrieval
and  of  course this pattern of expression does not appear  always,only in
some kind of materials. Give me please some ideas. Thanks to all.