I have used numerous types of paraffin and after more years think it is a
personal preference. Somewhat dependent on the type of tissue you are
cutting. Harder paraffin for harder tissue. Most of my knife marks are
caused by overly dry tissue or small bits of bone in the case of brain. I am
currently using paraffin from Shandon but have used Surgipath, paraplast and
a number of others. Biggest challenge on changing [my opinion only] is
getting the temp of cold plate and water bath optimal.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317



-----Original Message-----
From: Martin, Ronald [mailto:Ronald.Martin@umassmed.edu]
Sent: Tuesday, December 10, 2002 9:09 AM
To: histonet@pathology.swmed.edu
Subject: paraffin and microtome blades for mouse tissue


I was wondering what type of paraffin  research techs would suggest for
mouse histo? Also, what types of blades section better with mouse tissue?
I'm currently getting a lot of knife marks in my sections. All suggestions
are appreciated.
Thanks,
Ron