RE: brain question
>I think the problem is in the initial freezing of the brains. Ice crystals
>will grow if the tissue is not frozen fast enough i.e. snap frozen in liquid
>nitrogen.
Better in an isopentane slurry in LN2. The isopentane has less of an
insulating effect when it evaporates than LN2 alone. The best way of
course is to use a brain blower which can give homogenous water ice.
Bill
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____________________
Wm F Blank MD
Heartland Laboratory, Inc
Chaffee, MO 63740
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