Fwd:nonalchohol dehydration?
>Hello Histonetters, This question is being asked by one of our
>faculty. Any suggestions are welcome. Thank you in advance and have
>a great weekend! John
>
>subject: dehydration without alcohol
>
>We are looking for a way to dehydrate small trabecular bone struts
>(approx. 100-200 microns diameter, 2 mm long) without using
>alcohols. The trabecular bone struts are vitally stained with
>various fluorescent dyes, and ethanol has, in the past, caused us to
>lose the dyes. The struts will be fixed in PFA, then need to be
>dehydrated in order to allow embedding in PMMA.
--
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635
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