I stained with of my section on two different slide type from the same 
block. It happend that one is negative and the other is positive. Also, all 
the slide was moist at all time.

Thao


>From: rueggp 
>To: Thu-Thao Pham 
>CC: Bonnie.P.Whitaker@uth.tmc.edu, histonet@pathology.swmed.edu
>Subject: Re: Plus slides
>Date: Wed, 12 Dec 2001 13:51:29 -0700
>
>It is not the slide that is giving you negative staining unless the 
>reagents
>rolled off or dryed up on the ProbeOn slide, I would think there was 
>another
>problem causing the neg. result and you are focusing on the slide because 
>that
>was what was most obvious as different.
>Patsy Ruegg
>
>Thu-Thao Pham wrote:
>
> > Hi everyone,
> >
> > Thank you to everyone for your response to questions about the 
>microscrope
> > plus slides.
> >
> > I still don't understand why the section on the ProbeOn slide give me a
> > negative stainging whereas my regular superfrosted/plus slide give me a
> > positive staining.
> >
> > I have always use a plus slides on all my section. It was only at that 
>time
> > and day when I ran out of the superfrosted/plus slides and we had so 
>many of
> > the ProbeOn charged slide boxes hanging around and I happened to use it 
>on
> > my section until we get the regular superfrosted/plus slides deliver to 
>us.
> >
> > Thao
> >
> > _________________________________________________________________
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