-----Original Message-----
From: HistoNet Server <histonet@pathology.swmed.edu>
To: HistoNet Server <histonet@pathology.swmed.edu>
Date: Wednesday, December 20, 2000 1:03 AM
Subject: Daily Digest


>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 09:39:45 -0600
>From: Philip Oshel <peoshel@facstaff.wisc.edu>
>Subject: Fwd: Vibratome sectioning advice
>
>I used to cut fish brains on a Vibratome. They were embedded in 18%
>gelatin and stuck to the stage with gap-filling superglue. The
>"gap-filling" was important -- ordinary superglue didn't hold well
>enough.
>
>Phil
>
>>Dear Histonetters,
>>
>>A further point to ponder.
>>I am keen to cut some small ganglia on a vibrotome (40-50 micron)
>>in preparation for confocal fluorescent microscopy and need to
>>maximise my section count. I there a semi-rigid support or
>>embedding media that would allow me to mount these flat, soft
>>ganglia to successfully section in transverse rather than
>>longitudinal ?
>>
>>I am considering a concentrated agar solution in which to embed,
>>but conscious of needing to retain the ability to secure the base of
>>any block to the chuck using supa-glue.
>>
>>Any takers ? Thanks in advice.
>>
>>Cheers,
>>
>>Richard L. Young, PhD
>>
>>Nerve-Gut Research Laboratory
>>Royal Adelaide Hospital
>>Level 1, Hanson Centre
>>Frome Road
>>Adelaide, South Australia 5000
>>Phone: +61 8 8222 2077
>>Fax: +61 8 8222 5934
>>E-mail: ryoung2@mail.rah.sa.gov.au
>
>- --
>*** Be famous! Send a Tech Tip or article to Microscopy Today! ***
>Philip Oshel
>Technical Editor, Microscopy Today
>P.O. Box 620068
>Middleton, WI  53562-0068
>Voice: days (608) 263-4162, evening (608) 833-2885
>Fax (608) 836-1969  (please make sure my name is on any fax)
>
>Address for UPS, FedEx, or other couriers:
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI   53706
>fax: (608) 262- (dept. fax)
>
>peoshel@facstaff.wisc.edu
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 09:40:05 -0600
>From: "Kim Kusser" <kkusser@trudeauinstitute.org>
>Subject: Re: mouse mammary tumor Ab
>
>
>
>Are you staining tissues with suspected tumors caused by the mouse mammary
>tumor VIRUS (MMTV) or just any type of mouse mammary tumor?
>
>Kim
>
>
>
>
>Date: 14 Dec 2000 11:32:41 -0600
>From: "Jan Shivers" <shive003@maroon.tc.umn.edu> (by way of Histonet)
>Subject: Re: mouse mammary tumor Ab
>
>Chris (and any one else who might have some information),
>
>I guess I should have been more clear in my request for mouse mammary tumor
>Abs.  Yes, I assume that human cytokeratin Abs will cross-react with the
>mouse tissue.  They have done so on all other species that I've used them
on
>here in the Vet Diag Lab.  What I need is an antibody specific for mammary
>tumor, so that we can differentiate these tumors from salivary gland
tumors,
>etc.  I need to be able to say that it is more than just an epithelial
>tumor, or even more than a glandular or ductual epithelial tumor.  Is there
>such a beast that will work in mouse tissue?
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 10:15:58 -0600
>From: "Harvey, Jennifer" <Jennifer_Harvey@URMC.Rochester.edu>
>Subject: RE: EDTA as Decalcifying Agent?
>
>Gayle,
>
>Could you tell me more about IMEB. I make my own 10% EDTA and we use a ton
>of it. I am interested in finding some place to make it to my
>specifications.
>
>Thanks
>
>Jennifer Harvey
>University of Rochester
>Department of Ortho/Path
>Rochester NY
>716-273-4129
>
>> ----------
>> From: Gayle Callis
>> Sent: Monday, December 18, 2000 8:55 AM
>> To: HOOVER_JENNIFER@LILLY.COM; ANATECH LTD; histonet@pathology.swmed.edu
>> Subject: Re: EDTA as Decalcifying Agent?
>>
>> At 01:27 PM 12/18/00 -0500, you wrote:
>> >        Hello Histonetters,
>> >
>> >                I have  a few questions regarding EDTA as a decalcifying
>> >agent.  1)  why is it not more commercialized (ready-made) which leads
to
>>
>> >question
>>
>> You can buy it commercially prepared, Poly Scientific, Newcomer Supply,
>> Rowley Biochemical, probably Decal Corp have availability.  I think IMEB
>> will custom produce a decalcifier to your specifications.
>>
>>
>> 2)  when I prepare EDTA in the laboratory what is the
>> >approximate shelf life?
>>
>> MSDS may help here, I think it is very stable in water, with PBS,
>> something
>> might grow?? but as in all things, expiration dates can, will and should
>> be
>> given, you would probably use it up before expiration date anyway.
>>
>> I have read my histology books and found
>> >reference to EDTA but I know the veteran histonetters and bone
>> researchers
>> >are probably proficient with knowledge on this particular subject.
Also,
>>
>> >are there any good reference articles out there dealing with
decalcifying
>>
>> >agents and effects on tissue?
>>
>> Sorry folks! Tooting horn a tidge, Callis and Sterchi, Decalcification of
>> bone;  literature review and practical study of various decalcifying
>> agents, methods and their effects on bone histology  J of
Histotechnology,
>> 21(1):49-58, 1998 with extensive referencing on effects, IHC and
>> proteoglycan work, etc.  Happy to send original reprint.
>>
>> I also need some feedback on how important
>> >is it to rinse the tissue in running tap water after acid
decalcification
>>
>> >to remove acid residue before processing?  This has been a topic of
>> >debate.
>>
>> Rinse! I tried debating it, and decided rinse was a better choice,
>> particularly large bones.   Usually an hour to 4 hours, bone slabs, 3 - 5
>> mm thick, smaller- less time, and with EDTA at must, or a ppt forms when
>> it
>> comes in contact with alcohol, crunchy sectioning (Yuck!)
>>
>>  The main thing is to STOP decalcification, rinsing helps do just that,
>> then into 70% alcohol (Culling recommended 2 or 3 changes of alcohol
>> rinses
>> to get rid of acid, and the alcohol stops the decalcification action).
>> Long overnight rinses can create swelling, however, residual acid will
>> continue protein hydrolysis after calcium removal, leading  to ROTTEN
>> nuclear/soft tissue staining/damage.   Better to err with some swelling
>> than ruin precious staining characteristics and antigens.  You can
>> neutralize acid with several changes of saturated lithium carbonate or
>> sodium bicarbonate, test with litmus paper to neutrality, rinsing is
>> cheaper and easy.  Small bone biopsies are often plunked into processor
>> without problems. Large or numerous bones, acid decalcified contaminate
>> reagents, be prepared to change these more often if you don't rinse or
>> risk
>> ruining other processed tissues.  Debated for years, lost arguement and
>> rinse faithfully.
>>
>>
>> Gayle Callis
>> Veterinary Molecular Biology
>> Montana State University
>> Bozeman MT 59717-3610
>> 406 994-4705
>> 406 994-4303
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 10:16:22 -0600
>From: Philip Oshel <peoshel@facstaff.wisc.edu>
>Subject: virus warning
>
>Listers,
>
>This virus warning just came through our department, sent by
>Microsoft. I haven't found any notice of this virus on the CERT,
>CIAC, or MacAfee sites -- virus alerts or hoaxes, but none of them
>have an update newer than 14 December. I haven't found it on the
>Microsoft or IBM sites, either, but these aren't particularly easy to
>navigate. If these viruses are as serious as reported, I'd be careful
>until it's known whether or not this warning is true, or another hoax.
>
>Phil
>
>>URGENT WARNING FROM MICROSOFT
>>
>>  >>1.  There is a new virus - WOBBLER. It will arrive on e-mail titled
>>"CALIFORNIA."
>>IBM and AOL have announced that it is very powerful, more so than Melissa;
>>there is no remedy. It will eat all your information on the hard drive and
>>also destroys Netscape Navigator and Microsoft Internet Explorer. Do not
>>open anything with this title and please pass this message on to all your
>>contacts and anyone who uses your e-mail facility.  Not many people seem
to
>>know about this yet so propagate it as fast as possible.
>>  >>2.  If you receive an e-mail titled "Win A Holiday" DO NOT open it.
It
>>will erase everything on your hard drive. Forward this letter to as many
>>people as you can.  This is a new, very malicious virus.
>- --
>}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
>Philip Oshel
>Supervisor, AMFSC and BBPIC microscopy facilities
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI  53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 10:57:42 -0600
>From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
>Subject: STOP out of office replies when on vacation
>
>Dear Histonetters,
>
>Time for holiday vacation is here.  STOP, CEASE the UNWELCOME out of office
>replies. They are useful for your inhouse/personal emailing, but do not
>need to be on Histonet again, and again, and again - ad
nauseum!!!!!#$!!$@!#$
>
>UNSUBSCRIBE from Histonet!!!  then subscribe when you get back, then
>Histonet will send a lists of How T0's - put in hard copy for "when in
>doubt, follow directions" PLEASE!
>
>Your automatic out of office replies are junk mail, the endless stream of
>nothing, nothing, nothinggggggggggggggggggg-------
>
>Thanks from one Bah Humbug, AM I SHOUTING!!!  You betcha!
>
>
>
>
>
>
>
>
>Gayle Callis
>Veterinary Molecular Biology
>Montana State University
>Bozeman MT 59717-3610
>406 994-4705
>406 994-4303
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 10:58:10 -0600
>From: Debbie Pepperal <dpeppera@mail.newcastle.edu.au>
>Subject: Re: pathology photography
>
>
>Hi Terry
>
>The routine Pathology lab here has the  Histovision Digital Imaging System
>from Milestone Laboratory Systems. if you need any more information; let me
>know.
>As we are in the research labs, we donot use it often.
>
>Cheers
>
>Deb
>
>
>At 08:25 AM 18/12/2000 +0000, you wrote:
>> Does anyone know of an automated system of recording gross
>>pathology (dissection) for future reference, i.e. photography of the
>>specimen which may be archived with the Histology.
>>I am sure there is one up and running called the "picsie mouse"?
>>but cannot find any reference to this.
>>I have been of the Histonet for some time now so this may have
>>been discussed.
>>I see that the most difficult word to spell is still UNSUBSCRIBE!
>>Many thanks,
>>Terry.
>>Terry Hacker,
>>Medical Research Council,
>>Harwell,
>>Didcot,
>>Oxfordshire, OX11 ORD
>>01235 834393 x360
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 11:43:12 -0600
>From: RSRICHMOND@aol.com
>Subject: Re: virus warning
>
>Aw, c'mon, Phil Oshel at the U of Tyrophagy -
>
>The "California" and "Wobbler" virus spams are as old as the hills, as the
>Web goes. This variant claims origin at Microsoft (they used to come from
>IBM) - wonder when one of these warnings will claim to originate in the
>Vatican.
>
>The first virus hoax Web site I checked informed me - at -
>http://www.Vmyths.com/hoax.cfm?id=162&page=3
>
>>>Wobbler virus
>A typical variation on the original Good Times hoax virus alert (see
related
>ink). It claims your computer will suffer a horrible death if you receive
an
>email with the subject line "California" or with an attachment named
>"California".
>Like most hoax alerts, it urges you to forward it to others because "not
many
>people seem to know about this yet." It also claims IBM and AOL declared
>Wobbler more dangerous and more powerful than Melissa. Naturally, the hoax
>alert claims "there is no remedy" available if your computer gets
infected.<<
>
>PLEASE don't spam the list with virus warnings - well, unless the Ebola
virus
>breaks out in your lab, anyway!
>
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 11:44:09 -0600
>From: "Jim Manavis" <jim.manavis@imvs.sa.gov.au>
>Subject:
>
>I have heard mention about using saponin for cytokine immunohistochemistry
>(ie. Interleukins, etc) on formaldehyde fixed paraffin embedded material.
>Has anyone more information. Is it used in the wash buffer or retrieval
>buffer.
>
>Thanks
>
>Jim Manavis
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 12:30:03 -0600
>From: HOOVER_JENNIFER@LILLY.COM
>Subject: RE: EDTA as Decalcifying Agent?
>
>
>        Hi Gayle!  I would also very much appreciate the information!
>Thanks!
>
>
>Regards,
>
>Jennifer Hoover
>Eli Lilly and Company
>
>
>******************* NOTE *******************
>There may be important message content
>contained in the following MIME Information.
>********************************************
>
>
>- ------------------ MIME Information follows ------------------
>
>This is a multipart message in MIME format.
>- --=_alternative 00620530052569BA_=
>Content-Type: text/plain; charset="us-ascii"
>
><<<<<< See above "Message Body" >>>>>>
>
>- --=_alternative 00620530052569BA_=
>Content-Type: text/html; charset="us-ascii"
>
>
><br><font size=2 face="sans-serif">        Hi Gayle!
> I would also very much appreciate the information! Thanks!</font>
><br>
><br>
><br><font size=2 face="sans-serif">Regards,</font>
><br>
><br><font size=2 face="sans-serif">Jennifer Hoover</font>
><br><font size=2 face="sans-serif">Eli Lilly and Company</font>
>- --=_alternative 00620530052569BA_=--
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 12:30:42 -0600
>From: Terry.Marshall@rgh-tr.trent.nhs.uk
>Subject: RE: virus warning
>
>The language is typical of a hoax.
>
>'"Eat" everything ..... '
>'propagate it as fast as possible'
>'Forward this letter to as many people as you can.'
>
>Sent by Microsoft but not on the Microsoft site!
>Yeah.
>
>Terry L Marshall
>Histopathologist
>Rotherham General Hospital, Yorkshire
>
>
>- -
>
>
>Listers,
>
>This virus warning just came through our department, sent by
>Microsoft. I haven't found any notice of this virus on the CERT,
>CIAC, or MacAfee sites -- virus alerts or hoaxes, but none of them
>have an update newer than 14 December. I haven't found it on the
>Microsoft or IBM sites, either, but these aren't particularly easy to
>navigate. If these viruses are as serious as reported, I'd be careful
>until it's known whether or not this warning is true, or another hoax.
>
>Phil
>
>>URGENT WARNING FROM MICROSOFT
>>
>>  >>1.  There is a new virus - WOBBLER. It will arrive on e-mail titled
>>"CALIFORNIA."
>>IBM and AOL have announced that it is very powerful, more so than Melissa;
>>there is no remedy. It will eat all your information on the hard drive and
>>also destroys Netscape Navigator and Microsoft Internet Explorer. Do not
>>open anything with this title and please pass this message on to all your
>>contacts and anyone who uses your e-mail facility.  Not many people seem
to
>>know about this yet so propagate it as fast as possible.
>>  >>2.  If you receive an e-mail titled "Win A Holiday" DO NOT open it.
It
>>will erase everything on your hard drive. Forward this letter to as many
>>people as you can.  This is a new, very malicious virus.
>- --
>}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
>Philip Oshel
>Supervisor, AMFSC and BBPIC microscopy facilities
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI  53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 12:31:10 -0600
>From: Philip Oshel <peoshel@facstaff.wisc.edu>
>Subject: Re: virus warning
>
>Well, the sites I do have urls for did not list these titles,
>including under hoaxes. CERT and CIAC are the main computer security
>clearing houses. I got caught, I admit it. But spam? That's rather
>hostile.
>
>Thanks for the url, though.
>
>Phil
>
>>Aw, c'mon, Phil Oshel at the U of Tyrophagy -
>>
>>The "California" and "Wobbler" virus spams are as old as the hills, as the
>>Web goes. This variant claims origin at Microsoft (they used to come from
>>IBM) - wonder when one of these warnings will claim to originate in the
>>Vatican.
>>
>>The first virus hoax Web site I checked informed me - at -
>>http://www.Vmyths.com/hoax.cfm?id=162&page=3
>>
>>>>Wobbler virus
>>A typical variation on the original Good Times hoax virus alert (see
related
>>ink). It claims your computer will suffer a horrible death if you receive
an
>>email with the subject line "California" or with an attachment named
>>"California".
>>Like most hoax alerts, it urges you to forward it to others because "not
many
>>people seem to know about this yet." It also claims IBM and AOL declared
>>Wobbler more dangerous and more powerful than Melissa. Naturally, the hoax
>>alert claims "there is no remedy" available if your computer gets
infected.<<
>>
>>PLEASE don't spam the list with virus warnings - well, unless the Ebola
virus
>>breaks out in your lab, anyway!
>>
>>Bob Richmond
>>Samurai Pathologist
>>Knoxville TN
>
>- --
>}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
>Philip Oshel
>Supervisor, AMFSC and BBPIC microscopy facilities
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI  53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 13:31:41 -0600
>From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
>Subject: Re:
>
>Used throughout after rehydration, from blocking endog peroxidase through
>substrate, diluents, rinses, blocking solutions, then regular buffer is
>used to close the membrane, and chromogen is applied/developed.
>I am not sure whether it saponin needs to be in retrieval solutions.  Need
>to check a protocol for that one.
>
>To have info on how saponin is used with frozens(would be the same for
>paraffin sections) go to R&D systems website, applications/staining
>procedures, they give the whole thing.
>
>Also Histonet had long discussions a couple of years ago on saponin, go the
>archives for past messaging on saponin and detergents.
>
>
>
>At 03:30 PM 12/19/00 +1030, you wrote:
>>I have heard mention about using saponin for cytokine immunohistochemistry
>>(ie. Interleukins, etc) on formaldehyde fixed paraffin embedded material.
>>Has anyone more information. Is it used in the wash buffer or retrieval
>>buffer.
>>
>>Thanks
>>
>>Jim Manavis
>>
>>
>>
>>
>>
>Gayle Callis
>Veterinary Molecular Biology
>Montana State University
>Bozeman MT 59717-3610
>406 994-4705
>406 994-4303
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 14:00:06 -0600
>From: Miriam Schroeder <vitalred@yahoo.com>
>Subject: Re: Bagels and LOX
>
>*Miriam is Laughing*
>
>- --- RSRICHMOND@aol.com wrote:
>> >>Does anybody know anything specifically about LOX
>> cells & a QUICK way to
>> stain them?<<
>>
>> The tough part is getting the bagels to adhere....
>>
>> Bob Richmond
>> Samurai Pathologist
>> Knoxville TN
>>
>
>
>__________________________________________________
>Do You Yahoo!?
>Yahoo! Shopping - Thousands of Stores. Millions of Products.
>http://shopping.yahoo.com/
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 14:00:36 -0600
>From: Denise Bland-Piontek <bland008@umn.edu>
>Subject: in situ question
>
>Does anyone know of a DNA specific probe for either the x or y
>chromosome? I've checked several suppliers of in situ probes to no
>avail, but no one seems to know if such probes exist or not? I've been
>asked by a researcher if either of these probes exist and will they work
>on paraffin embedded human tissue? Any advice is appreciated!
>Denise Bland-Piontek, HTL (ASCP)
>Scientist
>Anatomic Pathology Research Lab
>University of Minnesota
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 15:30:44 -0600
>From: "Laurie Colbert" <laurie.colbert@schs.com>
>Subject: Hacker Coverslipper
>
>I have been getting very tiny bubbles on my slides after coverslipping
>with the Hacker coverslipper.  I have tried to find an experienced rep
>who can come to the hospital and give me some tips, but I have not had
>much luck.   Is there anyone out there who can help me????  The
>pathologists are running out of patience!
>
>Laurie Colbert
>Huntington Memorial Hospital
>Pasadena, Ca
>(626) 397-5784
>
>
>
>----------------------------------------------------------------------
>
      Dear Greg,
             Our AP section has successfully used Meditech for 5 years. I
would pass along some tips though;
                     -be involved at implementation. You will have an
invaluable understanding of system capabilities
                     -Meditech, as well as other systems, have regional User
Groups. Much sharing of valuable information occurs amongst  the
histotechnologists and cytotechnologists who attend these meetings
                     -Use the system optimally. Medtitech has bar coded
pre-printed microscope label capability, as an example.  Ask for contact
information with laboratories currently using any features you may wish
implement.  As an end user, be an advocate for those  components that will
promote productivity and allow you work most efficiently.
                                                       Jeannette Ugorji,
Ht., Ct. leydelab@erols.com

>Date: 19 Dec 2000 15:50:04 -0600
>From: greg tesdall <gtesdall@yahoo.com>
>Subject: LIS
>
>Please. This has been covered when I wasn't in the
>market so with apologies I'm asking for information as
>we select a new LIS system for Anatomic Pathology and
>Cytology. I would like to hear of your best picks and
>also comments on the the Meditech system pathology
>module which our hospital is very high on but which we
>in Pathology have some, no many, reservations. We
>currently use Pathlab 3. Thanks,Greg in Nebraska.
>
>__________________________________________________
>Do You Yahoo!?
>Yahoo! Shopping - Thousands of Stores. Millions of Products.
>http://shopping.yahoo.com/
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 16:30:39 -0600
>From: "Histo-Scientific Research Laboratories" <histosci@shentel.net>
>Subject: Re: Hacker Coverslipper
>
>Dear Laurie,
>
>I am sorry to hear about your Hacker coverslipper.  I was having the same
>problems but Hacker take care of it immediately.  Have you tried calling
>1-800-4HACKER and speaking with Jim Mullen?  I am sure he will be very
>interested to hear of your problem and fix it ASAP.  Jim Mullen of Hacker
>took a 6+hour drive from NJ to fix our coverslippers on a days notice.
When
>he could not fix it, two days later he had three Meisei engineers fly in
>from Tokyo, Japan to fix them properly.  I can not thank Hacker enough for
>all of their help.  I have never had customer support as good as that of
>Hacker!  I am not a spokesman for the company, just a very satisfied
>customer!
>
>- -Tom Galati
>Histo-Scientific Research Labs.
>137 S. Main Street
>Woodstock, VA  22664
>(540)459-8211
>Fax: (540)459-8217
>tomgalati@hsrl.org
>- ----- Original Message -----
>From: "Laurie Colbert" <laurie.colbert@schs.com>
>To: "Histonet" <histonet@pathology.swmed.edu>
>Sent: Tuesday, December 19, 2000 2:38 PM
>Subject: Hacker Coverslipper
>
>
>> I have been getting very tiny bubbles on my slides after coverslipping
>> with the Hacker coverslipper.  I have tried to find an experienced rep
>> who can come to the hospital and give me some tips, but I have not had
>> much luck.   Is there anyone out there who can help me????  The
>> pathologists are running out of patience!
>>
>> Laurie Colbert
>> Huntington Memorial Hospital
>> Pasadena, Ca
>> (626) 397-5784
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 16:30:56 -0600
>From: "Cordova, Jean" <JCordova@omlabs.com>
>Subject: Vacancy rates
>
>
>Could someone post again the current vacancy rates for histotechnicians and
>histotechnologist?
>
>Jean Cordova, HT/HTL ASCP
>NPS PATHOLOGY
>ext 2137
>
>
>
>
>******************* NOTE *******************
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>contained in the following MIME Information.
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>
>
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><<<<<< See above "Message Body" >>>>>>
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><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN">
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><META NAME="Generator" CONTENT="MS Exchange Server version 5.5.2653.12">
><TITLE>Vacancy rates</TITLE>
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><P><FONT SIZE=2 FACE="Arial">Could someone post again the current vacancy
>rates for histotechnicians and histotechnologist?</FONT>
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><P><B><FONT FACE="Arial">Jean Cordova, HT/HTL ASCP</FONT></B>
><BR><B><FONT FACE="Arial">NPS PATHOLOGY</FONT></B>
><BR><B><FONT FACE="Arial">ext 2137</FONT></B>
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>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 17:30:09 -0600
>From: "Andrea Voogt" <ndrvgt@dordt.edu>
>Subject: 2000 ASCP SALARY SURVEY AND VACANCY RATES
>
>>URGENT WARNING FROM MICROSOFT
>>
>>  >>1.  There is a new virus - WOBBLER. It will arrive on e-mail titled
>>"CALIFORNIA."
>>IBM and AOL have announced that it is very powerful, more so than Melissa;
>>there is no remedy. It will eat all your information on the hard drive and
>>also destroys Netscape Navigator and Microsoft Internet Explorer. Do not
>>open anything with this title and please pass this message on to all your
>>contacts and anyone who uses your e-mail facility.  Not many people seem
to
>>know about this yet so propagate it as fast as possible.
>>  >>2.  If you receive an e-mail titled "Win A Holiday" DO NOT open it.
It
>>will erase everything on your hard drive. Forward this letter to as many
>>people as you can.  This is a new, very malicious virus.
>- --
>}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
>Philip Oshel
>Supervisor, AMFSC and BBPIC microscopy facilities
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI  53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 19 Dec 2000 20:15:50 -0600
>From: denise M m Long-Woodward <denisew2@juno.com>
>Subject: Shandon Consul
>
>W're in the market for a new coverslipper!  Hooray!
>Demo-ing the Shandon Consul right now and like it.  Could I hear from
>other "Consul" owners?
>Anyone have anyother recommendations?
>Thanks a bunch and Happy Holidays!
>Denise Long Woodward
>
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>Here are the messages received yesterday!
>