Dear Histonetters,

Barry Rittman wrote:
	As noted by several others here, the times for most techniques
are guides only
	to provide a range. The results depend on many  factors such as
different
	fixation and processing techniques and times, thickness of
section, type of
	tissue and final result that is desired.

I couldn't agree more.  As a vendor of microwave processors, I am always
being asked for fixation and processing schedules which are ideal for
all specimens under all possible conditions.  Individual labs need to
understand that they may have to experiment to optimize procedures, but
there is a growing resistance to this idea.  In my workshops, I try to
explain the principles behind the process so that trained technologists
will understand the impacts of varying certain parameters.

Best regards,
Steven Slap

*******************************************
Energy Beam Sciences, Inc.
The Laboratory Microwave Company
Adding Brilliance to Your Vision
http://www.ebsciences.com <http://www.ebsciences.com> 
*******************************************