A recent question about the staining time of hematoxylin alludes to the 
point of the recommended time in staining protocols.
This time is just that......that recommended by the author(s), but does not 
reflect what is required for your own conditions. The microscope is the 
ultimate control, and not what is written down in a cookbook-type method.
I have found that newer students to the profession tend to place too much 
emphasis on exact staining times and not on microscopic control.

Just my observation, but would appreciate comments.

Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish rite Hospital for Children
Dallas


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