Re: re-staining faded slides
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From: | "Andrea Kelly" <canislupus@albanyonline.net> |
To: | "Carla Aiwohi" <Carla_Aiwohi@usgs.gov>, <HistoNet@pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 20 Aug 1999 21:41:27 -0400 |
Content-Type: | text/plain; charset="iso-8859-1" |
I would take them back as you planned only go all the way to water.Then go
to 0.5-1% acid alcohol to destain (about 1-3 minutes). Wash in water then
rinse in distilled.Have you seen Charles Churukian's Staining Manual?
Besides all of the wonderful microwave methods there is a ton of useful
information such as destaining, shelf life of solutions/stains, how to get
specific stains out of clothing, hands and glassware. It is very reasonable
too.
Andrea Kelly
-----Original Message-----
From: Carla Aiwohi <Carla_Aiwohi@usgs.gov>
To: HistoNet@pathology.swmed.edu <HistoNet@pathology.swmed.edu>
Date: Friday, August 20, 1999 1:05 PM
Subject: re-staining faded slides
>Hi everyone,
>
> I have three faded slides that were stained with PAS, H&E and
Richardson's
>about 10 years ago. Can anyone offer advice as to the best way to
rejuvenate
>them? I had planned to soak them in xylene, take slides to 70% then
re-stain
>using our usual procedures. Thanks for your help and have a good weekend.
>
>Carla Aiwohi
>Western Fisheries Research Center
>Seattle, WA
>
>
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