Dear Lisa,

If you try to lower the cryochamber to minus 30 deg. C. this will enable you
to achieve full sections, and should overcome the effect of the fat in the
tissue that is causing you problems.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright@brightinstruments.com
Web Site: www.brightinstruments.com

++++++++++++++++++++++++++++++++++++++++++++++++
-----Original Message-----
From: Lisa Lafranco <llafranco@ucdavis.edu>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Friday, August 13, 1999 01:00
Subject: Cryostat sectioning of synovium


>Hi all,
>
>I am trying to cryosection canine synovium and am having difficulty.  The
>section comes up with holes where the tissue should be.  I believe this is
>due to the amount of fat in the tissue.  Does anyone have advice on how to
>handle this type of tissue?  The cryostat I am using is set at 25 degrees,
>and the sections are 8 microns.
>
>Thanks for your help,
>
>Forward response to llafranco@ucdavis.edu
>
>