Richard

Are you using any EtOH in treating your tissue before you mount the
sections on slides?  
We were having a similar problem just the other week.  Our frozen
sections were coming off the
slides poly L lysine or no.  The tissue had been fixed but held in 70%
EtOH until sectioning.
Once I stopped exposing the tissues to EtOH the sections stopped coming
off the slides.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Wed, 11 Aug 1999, Richard G. Lea wrote:

> To whoever can help,
> 
> I am attempting to carry out in-situ hybridisation on sections of tissue
> which have been cultured for 24 hours.  The control non-cultured tissues
> are fine but after culture the tissue sections fall off the slides
> during the in-situ protocol.  We have tried TESPA and double coated
> gelatin, PLL slides however the problem remains.  How can I stop these
> sections coming off the slides - any suggestions gratefully received.
> 
> Richard Lea
> -- 
> Richard G. Lea PhD
> Senior Research Scientist
> Division of Nutrition, Pregnancy and Development
> Rowett Research Institiute
> Greenburn Road
> Bucksburn
> Aberdeen AB21 9SB
> 
> Tel:  44(0) 1224 712751 x 2319
> Fax:  44(0) 1224 716622
>