Hi Donna,

The most commonly used silver stain for H. pylori is the Steiner, some
pathologists will request a Warthin-Starry but it really isn't meant
for this particular "bug".  The only other stain is a non-silver
method, Wolbach's Giemsa or a modified May-Grunwald.  Most hospitals
run it as a back-up diagnostic procedure for gastric biopsies.

The Steiner procedure can be found in Sheehan  or in Carson.  I'd go
with the Carson method it is much simpler and less time consuming.  The
decision to microwave depends on how comfortable the tech is and if
they want they can try both and see which they feel more comfortable
with.  Poly-Scientific does make a kit that many hospitals use, but you
may want to consider making the solutions for this procedure yourself,
silver stains are sensitive.

Vikki Baker


--- Donna Sitrin  wrote:
> I have an employee who is taking the HTL soon and he
> is required to do a
> silver stain for h. pylori.  Does anyone have any
> information on this?  We
> haven't found anything in our current resources.
> 
> Thank you,
> Donna Sitrin
> Unipath, Denver
> 
> 
> 

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