I use the NFkB p65 Santa Cruz (only one from them I do use) at 1:500 or .4ug/ml overnight and it has worked just fine. I recently tried the goat polymer from Biocare with the same antibody at 1:100,200,300,400, and 500 and still could go out a lot more.

"Mino-Kenudson, Mari,M.D."  wrote:  Hello, 

We are trying to optimize 2 NF kappa B antibodies for immunohistochemistry on
formalin-fixed, paraffin-embedded tissue.
They are NFkB p50 (clone: sc-114) and NFkB p65 (clone: sc-8008) produced by
Santa Cruz. 
We followed the instructions, but we only got very week signals.
Any suggestions would be greatly appreciated. 

Thank you,
Mari


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