I don't have a protocol for doing this on urine, but if I wanted to do
it, the first thing I'd try would be trypsanizing the cells after
spinning to obtain a pellet. Then I'd spin down again, decant the
remaining trypsan, re-suspend in PBS, and make cytospins. Then it's
happy FISHing!
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Mobile (702) 759-9229
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester
Raff
Sent: Monday, August 13, 2007 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FISHing trip
I haven't gotten any help from the pathologist listserve, and the
UroVysion FISH listserve doesn't have many users, so I am asking the
histotechs for help! Maybe you have friends doing FISH....
We are doing FISH studies on urine (UroVysion) and frequently run into
cell clumps/clusters. Since the architecture is not what we are
assessing via FISH, it would be useful to disrupt these clumps and
obtain single cells for analysis. Does anyone have a protocol for doing
this on urine specimens?
Thanks in advance.
Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, IL 60154
ph: 708-486-0076
fax: 708-486-0080
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"EMF " made the following annotations.
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